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Volume 27, Number 12—December 2021
Research

Surface‒Aerosol Stability and Pathogenicity of Diverse Middle East Respiratory Syndrome Coronavirus Strains, 2012‒2018

Neeltje van Doremalen1, Michael Letko1Comments to Author , Robert J. Fischer, Trenton Bushmaker, Jonathan Schulz, Claude K. Yinda, Stephanie N. Seifert, Nam Joong Kim, Maged G. Hemida, Ghazi Kayali, Wan Beom Park, Ranawaka A.P.M. Perera, Azaibi Tamin, Natalie J. Thornburg, Suxiang Tong, Krista Queen, Maria D. van Kerkhove, Young Ki Choi, Myoung-don Oh, Abdullah M. Assiri, Malik Peiris, Susan I. Gerber, and Vincent J. MunsterComments to Author 
Author affiliations: National Institutes of Health, Hamilton, Montana, USA (N. van Doremalen, M. Letko, R.J. Fischer, T. Bushmaker, J. Schulz, C.K. Yinda, S.N. Seifert, V.J. Munster); Washington State University, Pullman, Washington, USA (M. Leitko, S.N. Seifert); Seoul National University College of Medicine, Seoul, South Korea (N.J. Kim, W.B. Park, M.-d. Oh); King Faisal University, Al-Hasa, Saudi Arabia (M.G. Hemida); Kafrelsheikh University, Kafrelsheikh, Egypt (M.G. Hemida); University of Texas Health Sciences Center, Houston, Texas, USA (G. Kayali); University of Hong Kong, Hong Kong, China (R.A.P.M. Perera, M. Peiris); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A. Tamin, N.J. Thornburg, S. Tong, K. Queen, S.I. Gerber); World Health Organization, Geneva, Switzerland (M.D. van Kerkhove); Chungbuk National University, Cheongju City, South Korea (Y.K. Choi); Ministry of Health, Riyadh, Saudi Arabia (A.M. Assiri)

Main Article

Figure 2

Stability of MERS-CoV strains on surfaces and in aerosols compared with those for SARS-CoV-2. Simple linear regression of virus was used for different surfaces and in aerosols. For surface stability, 50 μL of MERS-CoV or SARS-CoV-2 was spread on a surface, either polypropylene, stainless steel, copper, or silver; 1 mL of Dulbecco’s modified Eagle medium was added at times 0, 1, 24, 48, or 72 hours, and samples were titrated. For aerosol stability, MERS-CoV‒ or SARS-CoV-2‒containing aerosols were sprayed into a Goldberg drum; samples were taken at times 0, 30, 60, 120, and 180 min and then titrated. Linear regression was calculated per virus and indicated as lines. Dotted lines indicate limits of detection. Strain sources are listed in the legend for Figure 1. MERS-CoV, Middle East respiratory syndrome coronavirus; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; TCID50, median tissue culture infectious dose.

Figure 2. Stability of MERS-CoV strains on surfaces and in aerosols compared with those for SARS-CoV-2. Simple linear regression of virus was used for different surfaces and in aerosols. For surface stability, 50 μL of MERS-CoV or SARS-CoV-2 was spread on a surface, either polypropylene, stainless steel, copper, or silver; 1 mL of Dulbecco’s modified Eagle medium was added at times 0, 1, 24, 48, or 72 hours, and samples were titrated. For aerosol stability, MERS-CoV‒ or SARS-CoV-2‒containing aerosols were sprayed into a Goldberg drum; samples were taken at times 0, 30, 60, 120, and 180 min and then titrated. Linear regression was calculated per virus and indicated as lines. Dotted lines indicate limits of detection. Strain sources are listed in the legend for Figure 1. MERS-CoV, Middle East respiratory syndrome coronavirus; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; TCID50, median tissue culture infectious dose.

Main Article

1These authors contributed equally to this article.

Page created: August 26, 2021
Page updated: November 19, 2021
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