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Volume 27, Number 12—December 2021
Research

Surface‒Aerosol Stability and Pathogenicity of Diverse Middle East Respiratory Syndrome Coronavirus Strains, 2012‒2018

Neeltje van Doremalen1, Michael Letko1Comments to Author , Robert J. Fischer, Trenton Bushmaker, Jonathan Schulz, Claude K. Yinda, Stephanie N. Seifert, Nam Joong Kim, Maged G. Hemida, Ghazi Kayali, Wan Beom Park, Ranawaka A.P.M. Perera, Azaibi Tamin, Natalie J. Thornburg, Suxiang Tong, Krista Queen, Maria D. van Kerkhove, Young Ki Choi, Myoung-don Oh, Abdullah M. Assiri, Malik Peiris, Susan I. Gerber, and Vincent J. MunsterComments to Author 
Author affiliations: National Institutes of Health, Hamilton, Montana, USA (N. van Doremalen, M. Letko, R.J. Fischer, T. Bushmaker, J. Schulz, C.K. Yinda, S.N. Seifert, V.J. Munster); Washington State University, Pullman, Washington, USA (M. Leitko, S.N. Seifert); Seoul National University College of Medicine, Seoul, South Korea (N.J. Kim, W.B. Park, M.-d. Oh); King Faisal University, Al-Hasa, Saudi Arabia (M.G. Hemida); Kafrelsheikh University, Kafrelsheikh, Egypt (M.G. Hemida); University of Texas Health Sciences Center, Houston, Texas, USA (G. Kayali); University of Hong Kong, Hong Kong, China (R.A.P.M. Perera, M. Peiris); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A. Tamin, N.J. Thornburg, S. Tong, K. Queen, S.I. Gerber); World Health Organization, Geneva, Switzerland (M.D. van Kerkhove); Chungbuk National University, Cheongju City, South Korea (Y.K. Choi); Ministry of Health, Riyadh, Saudi Arabia (A.M. Assiri)

Main Article

Figure 3

Middle East respiratory syndrome coronavirus replication in Vero E6 cells (A) and human airway epithelium (B). Replication is shown as geometric means; error bars indicate SDs. Vero E6 cells were infected with a multiplicity of infection of 0.01, and human airway epithelium were infected with a multiplicity of infection of 0.1. Samples of supernatants were obtained at 8, 24, 48 and 72 hours postinoculation and titrated. Statistically significant differences compared with those for the prototypical strain, EMC/12, were calculated by using ordinary 1-way analysis of variance, followed by a Bonferroni multiple comparisons test. Dotted lines indicate limits of detection. Strain sources are listed in the legend for Figure 1. TCID50, median tissue culture infectious dose. *p<0.05; **p<0.01.

Figure 3. Middle East respiratory syndrome coronavirus replication in Vero E6 cells (A) and human airway epithelium (B). Replication is shown as geometric means; error bars indicate SDs. Vero E6 cells were infected with a multiplicity of infection of 0.01, and human airway epithelium were infected with a multiplicity of infection of 0.1. Samples of supernatants were obtained at 8, 24, 48 and 72 hours postinoculation and titrated. Statistically significant differences compared with those for the prototypical strain, EMC/12, were calculated by using ordinary 1-way analysis of variance, followed by a Bonferroni multiple comparisons test. Dotted lines indicate limits of detection. Strain sources are listed in the legend for Figure 1. TCID50, median tissue culture infectious dose. *p<0.05; **p<0.01.

Main Article

1These authors contributed equally to this article.

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Page updated: November 19, 2021
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