Figure 4. Phylogenetic analysis of canine enteric coronavirus strains, including locations were sequences were obtained, in investigation of dogs with vomiting, United Kingdom. Trees are based on nucleotide sequences for M-gene (final alignment 299 positions) (A) and whole genome (final alignment 26,564 positions) (B). Evolutionary analysis was performed using the neighbor-joining method. Bootstrap testing using 1,000 replicates was applied; only values >70 are indicated. Sequences identified in this study are indicated in blue (strain 1), red (strain 2), and green (strain 3). Asterisks (*) indicate samples from animals meeting the case definition. Each phylogeny included closest matches in GenBank, as well as representative published canine coronavirus, feline coronavirus, and transmissible gastroenteritis virus isolates. Scale bars indicate substitutions per site. C) Approximate geographic location of sequences obtained in this study, number- and color-matched to sequences shown in panels A and B.