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Volume 27, Number 2—February 2021
Dispatch

Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

Catherine A. Hogan, ChunHong Huang, Malaya K. Sahoo, Hannah Wang, Becky Jiang, Mamdouh Sibai, Marisa Holubar, Roshni Mathew, James Zehnder, and Benjamin A. PinskyComments to Author 
Author affiliations: Stanford University School of Medicine, Stanford, California, USA (C.A. Hogan, C. Huang, M.K. Sahoo, H. Wang, M. Holubar, R. Mathew, J. Zehnder, B.A. Pinsky); Stanford Health Care, Stanford (C.A. Hogan, B. Jiang, M. Sibai, B.A. Pinsky)

Main Article

Figure 2

Deming regression analysis of Ct values by strand-specific real-time reverse transcription PCR as a function of the Ct values by standard real-time reverse transcription PCR for severe acute respiratory syndrome coronavirus 2. Results of PCR for plus strand (A; y = 0.91x + 3.26) and minus strand (B; y = 0.88x + 17.30). Ct, cycle threshold.

Figure 2. Deming regression analysis of Ct values by strand-specific real-time reverse transcription PCR as a function of the Ct values by standard real-time reverse transcription PCR for severe acute respiratory syndrome coronavirus 2. Results of PCR for plus strand (A; y = 0.91x + 3.26) and minus strand (B; y = 0.88x + 17.30). Ct, cycle threshold.

Main Article

Page created: December 07, 2020
Page updated: January 24, 2021
Page reviewed: January 24, 2021
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