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Volume 27, Number 3—March 2021
Research

Genomic Characterization of hlyF-positive Shiga Toxin–Producing Escherichia coli, Italy and the Netherlands, 2000–2019

Federica GigliucciComments to Author , Angela H.A.M. van Hoek, Paola Chiani, Arnold Knijn, Fabio Minelli, Gaia Scavia, Eelco Franz, Stefano Morabito, and Valeria Michelacci
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (F. Gigliucci, P. Chiani, A. Knijn, F. Minelli, G. Scavia, S. Morabito, V. Michelacci); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (A.H.A.M. van Hoek, E. Franz)

Main Article

Figure 1

Whole-genome comparison of pR444_A–like plasmids in Shiga toxin–producing Escherichia coli strains harboring extraintestinal pathogenic E. coli (ExPEC)–associated virulence genes, Italy, 2000–2019. The pR444_A plasmid from RDEx444 strain was used as reference for alignment and gene annotation. Genomic annotation was performed by using the Prokka tool 1.14.5 (https://github.com/tseemann/prokka) and a multi-fasta file of trusted proteins related to ExPEC-associated genes on pR444_A. The comparative analysis also included the pS88 plasmid (GenBank accession no. CU928146.1) commonly found in ExPEC strains.

Figure 1. Whole-genome comparison of pR444_A–like plasmids in Shiga toxin–producing Escherichia coli strains harboring extraintestinal pathogenic E. coli (ExPEC)–associated virulence genes, Italy, 2000–2019. The pR444_A plasmid from RDEx444 strain was used as reference for alignment and gene annotation. Genomic annotation was performed by using the Prokka tool 1.14.5 (https://github.com/tseemann/prokka) and a multi-fasta file of trusted proteins related to ExPEC-associated genes on pR444_A. The comparative analysis also included the pS88 plasmid (GenBank accession no. CU928146.1) commonly found in ExPEC strains.

Main Article

Page created: December 23, 2020
Page updated: February 21, 2021
Page reviewed: February 21, 2021
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