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Volume 27, Number 4—April 2021
Dispatch

Stability of SARS-CoV-2 RNA in Nonsupplemented Saliva

Isabel M. Ott1, Madison S. Strine1, Anne E. Watkins, Maikel Boot, Chaney C. Kalinich, Christina A. Harden, Chantal B.F. Vogels, Arnau Casanovas-Massana, Adam J. Moore, M. Catherine Muenker, Maura Nakahata, Maria Tokuyama, Allison Nelson, John Fournier, Santos Bermejo, Melissa Campbell, Rupak Datta, Charles S. Dela Cruz, Shelli F. Farhadian, Albert I. Ko, Akiko Iwasaki, Nathan D. Grubaugh2Comments to Author , Craig B. Wilen2, Anne L. Wyllie2Comments to Author , and the Yale IMPACT Research team3
Author affiliations: Yale School of Public Health, New Haven, Connecticut, USA (I.M. Ott, A.E. Watkins, C.C. Kalinich, C.A. Harden, C.B.F. Vogels, A. Casanovas-Massana, A.J. Moore, M.C. Muenker, M. Nakahata, A.I. Ko, N.D. Grubaugh, A.L. Wyllie); Yale School of Medicine, New Haven (M.S. Strine, M. Boot, M. Tokuyama, A. Nelson, J. Fournier, S. Bermejo, M. Campbell, R. Datta, C.S. Dela Cruz, S.F. Farhadian, A.I. Ko, A. Iwasaki, C. Wilen); Howard Hughes Medical Institute, New Haven (A. Iwasaki)

Main Article

Table

Possible saliva collection devices for severe acute respiratory virus coronavirus 2 RNA testing

Tube type Collection Buffer type Cost per sample, USD Manufacturer
Oragene•Dx collection device (OGD-510)*
Funnel
Ethanol <24%; Tris 1%–5% (host DNA stabilization)
28.00
Genotek, https://www.dnagenotek.com
Samplify SD-3000
Funnel
Dry preservative; sodium dodecyl sulfate <1%
24.00
Samplify (URL not available)
Saliva collection kit
Funnel
Unknown
22.47
IBI Scientific, https://www.ibisci.com
SDNA-1000 small tubes*
Wide-mouth tube
Ethanol 10%–25%; 
Tris 1%–5%; thiocyanic acid:guanidine (1:1) 
25%–50%; pH 7.9–8.3
17.99
Spectrum Solutions, https://spectrumsolution.com
Saliva RNA Collection and Preservation Device
Wide-mouth tube
Unknown liquid, colorless, odorless
18
Norgen (Biotek), https://norgenbiotek.com
Liquid biopsy/spit devices
Complicated unit (various)
Unknown
9–12 each
Oasis Diagnostics, https://4saliva.com
OMNIgene•ORAL saliva collection device (OM-505)*
Funnel
Sodium dodecyl sulfate 
1%–5%; glycine,N,N′-trans- 1,2-cyclohexanediylbis
[N-(carboxymethyl)-,hydrate 
1%–5%; lithium chloride 0.5%–1.5%
9.50
Genotek
GeneFix Saliva DNA/RNA Collection
Funnel
Unknown liquid, colorless
9
Isohelix, https://isohelix.com
DNA/RNA Shield saliva collection kit*
Wide-mouth tube
Unknown liquid, colorless, 
pH 5.0–7
7.25
Zymo Research, https://www.zymoresearch.com
Saliva collection system
Small beaker
Unknown
Unavailable
Greiner Bio-One, ttps://www.gbo.com
Pedia•SAL Infant/Toddler Salivary Collection
Soother + collector
None
Unavailable
Oasis Diagnostics
Oral swab
Swab
None
1.76
Salimetrics, https://salimetrics.com
Saliva collection aid + cryovial
Straw + 2 mL collection vial
None
1.36/straw, 0.76/vial
Salimetrics
Urine collection cups
Wide-mouth cup
None
0.47
ThermoFisher, https://www.thermofisher.com
Sterile tube, large volume Wide-mouth tube None 0.46 (25 mL), 0.38 (5 mL) Eppendorf, https://www.eppendorf.com
Sterile tube, small volume Narrow-mouth tube None 0.16 (2 mL) ThermoFisher

*Approved by US Food and Drug Administration Emergency Use Authorization for saliva-based diagnostics.

Main Article

1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

3Team members team are listed at the end of this article.

Page created: February 09, 2021
Page updated: March 18, 2021
Page reviewed: March 18, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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