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Volume 27, Number 5—May 2021
Synopsis

Case Series of Laboratory-Associated Zika Virus Disease, United States, 2016–2019

Susan L. HillsComments to Author , Andrea Morrison, Shawna Stuck, Kayleigh Sandhu, Krystal L. Mason, Danielle Stanek, Julie Gabel, Matthew A. Osborne, Betsy A. Schroeder, Edhelene Rico, Cherie L. Drenzek, Glen R. Gallagher, Jennifer Fiddner, Lea A. Heberlein-Larson, Catherine M. Brown, and Marc Fischer
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (S.L. Hills, M. Fischer); Florida Department of Health, Tallahassee, Florida, USA (A. Morrison, D. Stanek); Georgia Department of Public Health, Atlanta, Georgia, USA (S. Stuck, J. Gabel, C.L. Drenzek); Massachusetts Department of Public Health, Jamaica Plain, Massachusetts, USA (K. Sandhu, M.A. Osborne, G.R. Gallagher, C.M. Brown); Pennsylvania Department of Health, Harrisburg, Pennsylvania, USA (K.L. Mason, B.A. Schroeder); Florida Department of Health in Miami-Dade County, Miami, Florida, USA (E. Rico); Allegheny County Health Department, Pittsburgh, Pennsylvania, USA (J. Fiddner); Florida Department of Health Bureau of Public Health Laboratories, Tampa, Florida, USA (L.A. Heberlein-Larson)

Main Article

Table

Laboratory results from 4 patients with laboratory-associated Zika virus disease, United States, 2016–2019*

Days post-onset of collection† Sample type Test conducted‡ Result
Case 1 1 Serum RT-PCR Zika virus RNA detected
2 Serum RT-PCR Zika virus RNA detected
2 Urine RT-PCR Zika virus RNA detected
2 Serum IgM ELISA Zika virus IgM equivocal
2
 Serum
 PRNT
 Zika virus titer >20, DENV titer <10
Case 2 4 Serum RT-PCR Negative
4 Urine RT-PCR Zika virus RNA detected
4 Serum IgM ELISA Zika virus IgM positive
4 Serum PRNT Zika virus titer <10, DENV titer <10
20 Serum RT-PCR Negative
20 Urine RT-PCR Negative
20 Serum IgM ELISA Zika virus IgM positive
20
 Serum
 PRNT
 Zika virus titer 320, DENV titer 20
Case 3 2 Serum RT-PCR Zika virus RNA detected
2 Serum IgM ELISA Negative
20 Serum RT-PCR Negative
20 Serum IgM ELISA Zika virus IgM positive
20 Serum PRNT Zika virus titer >1280, DENV titer <10
≈120
 Semen
 RT-PCR
 Zika virus RNA detected
Case 4 5 Serum RT-PCR Negative
5 Urine RT-PCR Zika virus RNA detected
5 Serum PRNT Zika virus titer 160
10 Urine RT-PCR Zika virus RNA detected
10 Serum RT-PCR Negative
10 Serum IgM ELISA Zika virus IgM positive
10 Serum PRNT Zika virus titer 1280

*CDC, Centers for Disease Control and Prevention; DENV, dengue virus; PRNT, 90% plaque-reduction neutralization test; RT-PCR, reverse transcription PCR. 
†Day 0 equals day of illness onset. 
‡Tests conducted at state public health laboratories, commercial laboratories, and CDC Arboviral Diseases Branch Reference Laboratory.

Main Article

Page created: March 13, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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