Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 5—May 2021
Research

Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses

Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin ZhouComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)

Main Article

Figure 5

Infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with spike G614 in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Vero E6, CV-1, A549, Mv1Lu, CRFK, MDCK-NBL-2, and MDCK-SIAT1 cell lines inoculated with MA/VPT1 at 5 × 105 TCID50/well in 12-well plates (MOI 1 to ≈5 depending on cell line). A) Supernatants collected at indicated times and used to determine viral replication kinetics by TCID50. B) Total viral RNA levels extracted from cells inoculated for the indicated times as determined by real-time reverse transcription PCR. Data are mean of n = 3 +SD. TCID50, median tissue culture infectious dose.

Figure 5. Infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with spike G614 in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Vero E6, CV-1, A549, Mv1Lu, CRFK, MDCK-NBL-2, and MDCK-SIAT1 cell lines inoculated with MA/VPT1 at 5 × 105 TCID50/well in 12-well plates (MOI 1 to ≈5 depending on cell line). A) Supernatants collected at indicated times and used to determine viral replication kinetics by TCID50. B) Total viral RNA levels extracted from cells inoculated for the indicated times as determined by real-time reverse transcription PCR. Data are mean of n = 3 +SD. TCID50, median tissue culture infectious dose.

Main Article

Page created: February 24, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external