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Volume 27, Number 5—May 2021
Research

Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses

Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin ZhouComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)

Main Article

Figure 8

MDCK-vector, MDCK-hACE2, and MDCK-cACE2 cells inoculated with USA-WA1 at 5 × 105 TCID50/well in 12-well plates in study of susceptibility to severe acute respiratory syndrome coronavirus 2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Supernatants collected at the indicated times. A) Viral titers determined by TCID50 assay; B) total viral RNA determined using real-time reverse transcription PCR (standard curve generated by synthetic RNA). Data for both panels are mean of n = 3 +SD. ACE, angiotensin-converting enzyme 2; cACE2, canine ACE2; hACE2, human ACE2; TCID50, median tissue culture infectious dose.

Figure 8. MDCK-vector, MDCK-hACE2, and MDCK-cACE2 cells inoculated with USA-WA1 at 5 × 105 TCID50/well in 12-well plates in study of susceptibility to severe acute respiratory syndrome coronavirus 2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Supernatants collected at the indicated times. A) Viral titers determined by TCID50 assay; B) total viral RNA determined using real-time reverse transcription PCR (standard curve generated by synthetic RNA). Data for both panels are mean of n = 3 +SD. ACE, angiotensin-converting enzyme 2; cACE2, canine ACE2; hACE2, human ACE2; TCID50, median tissue culture infectious dose.

Main Article

Page created: February 24, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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