Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 8—August 2021
Research

Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice

Illiassou Hamidou Soumana12, Bodo Linz13, Kalyan K. Dewan1Comments to Author , Demba Sarr, Monica C. Gestal4, Laura K. Howard5, Amanda D. Caulfield, Balázs Rada, and Eric T. Harvill
Author affiliation: University of Georgia, Athens, Georgia, USA

Main Article

Figure 1

Susceptibility of mice to colonization by Bordetella pertussis after treatment with enrofloxacin. A, B) C57BL/6 mice were pretreated 3 times intranasally with 45 µg enrofloxacin in 10 µL (blue squares) or with phosphate-buffered saline (PBS; red squares) before being challenged with 5 µL PBS containing (A) 500 CFU of B. pertussis; or (B) B. pertussis ranging from 10–10,000 CFU. Colonization was assessed 3 days post inoculation by enumerating the number of B. pertussis CFU recovered from nasal cavities. C) Colonization and growth of B. pertussis at 500 CFU after 0, 1, and 2 pretreatments with 45 mg of enrofloxacin. D) B. pertussis colonization after intranasal enrofloxacin pretreatment at various doses. Each square represents a single biologic replicate. Dotted lines indicate limit of detection. Horizontal bars indicate mean.

Figure 1. Susceptibility of mice to colonization by Bordetella pertussis after treatment with enrofloxacin. A, B) C57BL/6 mice were pretreated 3 times intranasally with 45 µg enrofloxacin in 10 µL (blue squares) or with phosphate-buffered saline (PBS; red squares) before being challenged with 5 µL PBS containing (A) 500 CFU of B. pertussis; or (B) B. pertussis ranging from 10–10,000 CFU. Colonization was assessed 3 days post inoculation by enumerating the number of B. pertussis CFU recovered from nasal cavities. C) Colonization and growth of B. pertussis at 500 CFU after 0, 1, and 2 pretreatments with 45 mg of enrofloxacin. D) B. pertussis colonization after intranasal enrofloxacin pretreatment at various doses. Each square represents a single biologic replicate. Dotted lines indicate limit of detection. Horizontal bars indicate mean.

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of British Columbia, Vancouver, British Columbia, Canada.

3Current affiliation: Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany.

4Current affiliation: Louisiana State University Health Science Center, Shreveport, Louisiana, USA.

5Current affiliation: Augusta University, Augusta, Georgia, USA.

Page created: June 16, 2021
Page updated: July 18, 2021
Page reviewed: July 18, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external