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Volume 27, Number 8—August 2021
Dispatch

Genomic Detection of Schmallenberg Virus, Israel

Adi BeharComments to Author , Omer Izhaki, Asael Rot, Tzvika Benor, Mario Yankilevich, Monica Leszkowicz-Mazuz, and Jacob Brenner
Author affiliations: Kimron Veterinary Institute, Beit Dagan, Israel (A. Behar, O. Izhaki, A. Rot, M. Leszkowicz-Mazuz, J. Brenner); Veterinary Field Services, Beit Dagan, Israel (T. Benor, M. Yankilevich)

Main Article

Table 1

Primer sets used for the amplification of Schmallenberg virus RNA-specific fragments of the S, medium M, and L segments by reverse transcription nested PCR*

Segment External primer sequence, 5′ → 3′ Internal primer sequence, 5′ → 3′ Expected product size, bp Reference
S
 AKAI206F: CAC AAC CAA GTG TCG ATC TTA S_nestF: TGG TTA ATA ACC ATT TTC CCC A 370
 External: (4); internal: this study
SimbuS637: GAG AAT CCA GAT TTA GCC CA
 S_nestR: GTC ATC CAY TST TCW GCA GTC A
M
 924F: CCG AAA ACA AGG AAA TTG TG 1899F: TAT AGT CCC TGG ATT AGG TC 430
 Forward primers: (8); reverse primers: this study
2331R: GGT TCA AAC ATC TCT AGG C
 2331R: GGT TCA AAC ATC TCT AGG C
L SNL_F: GCA AAC CCA GAA TTT GYW GA panOBV-L-2959 F: TTG GAG ART ATG ARG CTA ARA TGT G 370 External: this study; internal:
(6)
SNL_R: ATT SCC TTG NAR CCA RTT YC panOBV-L-3274R: TGA GCA CTC CAT TTN GAC ATR TC

*L, large; M, medium; S, small.

Main Article

References
  1. De Regge  N. Akabane, Aino and Schmallenberg virus-where do we stand and what do we know about the role of domestic ruminant hosts and Culicoides vectors in virus transmission and overwintering? Curr Opin Virol. 2017;27:15–30. DOIPubMedGoogle Scholar
  2. Sick  F, Beer  M, Kampen  H, Wernike  K. Culicoides biting midges—underestimated vectors for arboviruses of public health and veterinary importance. Viruses. 2019;11:376–94. DOIPubMedGoogle Scholar
  3. Brenner  J, Rotenberg  D, Jaakobi  S, Stram  Y, Guini-Rubinstein  M, Menasherov  S, et al. What can Akabane disease teach us about other arboviral diseases. Vet Ital. 2016;52:353–62.PubMedGoogle Scholar
  4. Hirashima  Y, Kitahara  S, Kato  T, Shirafuji  H, Tanaka  S, Yanase  T. Congenital malformations of calves infected with Shamonda virus, Southern Japan. Emerg Infect Dis. 2017;23:993–6. DOIPubMedGoogle Scholar
  5. Yanase  T, Murota  K, Hayama  Y. Endemic and emerging arboviruses in domestic ruminants in East Asia. Front Vet Sci. 2020;7:168. DOIPubMedGoogle Scholar
  6. Steyn  J, Motlou  P, van Eeden  C, Pretorius  M, Stivaktas  VI, Williams  J, et al. Shuni virus in wildlife and nonequine domestic animals, South Africa. Emerg Infect Dis. 2020;26:1521–5. DOIPubMedGoogle Scholar
  7. St George  TD, Standfast  HA. Simbu group viruses with teratogenic potential. In: Monath TP, editor. The arboviruses: epidemiology and ecology. Boca Raton (FL): CRC Press; 1989. p. 146–66.
  8. Hoffmann  B, Scheuch  M, Höper  D, Jungblut  R, Holsteg  M, Schirrmeier  H, et al. Novel orthobunyavirus in cattle, Europe, 2011. Emerg Infect Dis. 2012;18:469–72. DOIPubMedGoogle Scholar
  9. Yanase  T, Kato  T, Aizawa  M, Shuto  Y, Shirafuji  H, Yamakawa  M, et al. Genetic reassortment between Sathuperi and Shamonda viruses of the genus Orthobunyavirus in nature: implications for their genetic relationship to Schmallenberg virus. Arch Virol. 2012;157:1611–6. DOIPubMedGoogle Scholar
  10. Coupeau  D, Bayrou  C, Baillieux  P, Marichal  A, Lenaerts  AC, Caty  C, et al. Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses. Emerg Microbes Infect. 2019;8:381–95. DOIPubMedGoogle Scholar
  11. Golender  N, Brenner  J, Valdman  M, Khinich  Y, Bumbarov  V, Panshin  A, et al. Malformations caused by Shuni virus in ruminants, Israel, 2014–2015. Emerg Infect Dis. 2015;21:2267–8. DOIPubMedGoogle Scholar
  12. Brenner  J, Yanase  T, Kato  T, Yaakobi  S, Khinich  E, Paz  R, et al. Serological evidence suggests that several Simbu serogroup viruses circulated in Israel. Vet Ital. 2019;55:81–9.PubMedGoogle Scholar
  13. Behar  A, Rot  A, Lavon  Y, Izhaki  O, Gur  N, Brenner  J. Seasonal and spatial variation in Culicoides community structure and their potential role in transmitting Simbu serogroup viruses in Israel. Transbound Emerg Dis. 2020;67:1222–30. DOIPubMedGoogle Scholar
  14. Behar  A, Leibovich  BB, Edery  N, Yanase  T, Brenner  J. First genomic detection of Peaton virus in a calf with hydranencephaly in Israel. Vet Med Sci. 2019;5:87–92. DOIPubMedGoogle Scholar
  15. Kato  T, Shirafuji  H, Tanaka  S, Sato  M, Yamakawa  M, Tsuda  T, et al. Bovine arboviruses in Culicoides biting midges and sentinel cattle in southern Japan from 2003 to 2013. Transbound Emerg Dis. 2016;63:e160–72. DOIPubMedGoogle Scholar

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Page updated: July 18, 2021
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