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Volume 27, Number 8—August 2021

Replication in Human Intestinal Enteroids of Infectious Norovirus from Vomit Samples

Marie Hagbom, Jenny Lin, Tina Falkeborn, Lena Serrander, Jan Albert, Johan Nordgren, and Sumit SharmaComments to Author 
Author affiliations: Linköping University, Linköping, Sweden (M. Hagbom, J. Lin, L. Serrander, J. Nordgren, S. Sharma); Linköping University Hospital, Linköping (T. Falkeborn, L. Serrander); Karolinska University Hospital, Stockholm, Sweden (J. Albert); Karolinska Institutet, Stockholm (J. Albert)

Main Article


Details of the norovirus genotypes and titers in the 20 vomit samples tested for norovirus infectivity in human intestinal enteroids*

Sample name Genotype† Titer, GEq/mL‡
V1 GII.4§ 7.86 × 109
V2 GII.4 1.36 × 109
V3 GII.4 5.28 × 108
V5 GII.4 1.44 × 108
V6 GII.2 8.55 × 107
V8 GII.4 1.16 × 108
V11 GII.17 1.21 × 108
V12 GII.4 1.25 × 107
V18 GII.2 5.41 × 107
V19 GII.4 2.00 × 108
V20 GII.2 8.73 × 108
V21 GII.4 5.92 × 107
V22 GII.4 1.91 × 107
V23 GII.4 2.83 × 108
V24 GII.4 1.66 × 109
V25 GII.4 1.61 × 1010
V29 GII.4 1.51 × 108
V30 GII.4 8.91 × 106
V32 GII.4 9.55 × 107
V33 GII.4 3.52 × 108

*Bold indicates vomit samples that showed successful norovirus replication. GEq, genomic equivalent. †Norovirus genotype determined by partial sequencing of the VP1 gene encoding the major capsid protein. ‡Norovirus titer (GEq/mL) in undiluted vomit used to infect human intestinal enteroids. 100µL of 1:100 diluted sample was used as inoculum. §All GII.4 norovirus detected belonged to the GII.4 Sydney 2012 variant.

Main Article

Page created: July 15, 2021
Page updated: July 18, 2021
Page reviewed: July 18, 2021
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