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Development and Clinical Evaluation of a CRISPR-Based Diagnostic for Rapid Group B Streptococcus Screening

Lingxiao Jiang¹, Weiqi Zeng¹, Wanting Wu¹, Yingying Deng, Fusheng He, Wenli Liang, Mingyao Huang, Hong Huang, Yongjun Li, Xiaorui Wang, Hang Su, Shilei Pan

, and Teng Xu¹

Author affiliations: Zhujiang Hospital, Southern Medical University, Guangzhou, China (L. Jiang, Y. Deng, W. Liang, M. Huang); Vision Medicals Center for Medical Research, Shenzhen, China (W. Zeng, W. Wu, H. Huang, Y. Li, X. Wang, H. Su, T. Xu); Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China (W. Zeng, T. Xu); Zhujiang Hospital, Southern Medical University, Guangzhou (S. Pan)

Main Article

Positive and negative agreement of CRISPR-based diagnostic for rapid group B Streptococcus screening versus different reference standards*

Assay and result	CRISPR-GBS			% (95% CI)
	CRISPR-GBS			Sensitivity		Specificity		Positive predictive value		Negative predictive value
	Positive	Negative	Total	Sensitivity		Specificity		Positive predictive value		Negative predictive value
Direct culture
Positive	52	3	55		94.5 (83.9–98.6)		89.6 (85.9–92.5)		58.4 (47.5–68.6)		99.1 (97.1–99.8)
Negative	37	320	357
Total	89	323	412
PCR
Positive	54	3	57		94.7 (84.5–98.6)		90.1 (86.4–92.9)		60.7 (49.7–70.7)		99.1 (97.1–99.8)
Negative	35	320	355
Total	89	323	412
Direct culture and PCR
Positive	47	3	50		94.0 (82.5–98.4)		91.4 (87.9–94.0)		61.0 (49.2–71.7)		99.0 (97.1–99.8)
Negative	30	320	350
Total	77	323	400
Enriched culture
Positive	22	0	22		100 (81.5–100.0)		100 (62.9–100.0)		100 (81.5–100.0)		100 (62.9–100.0)
Negative	0	9	9
Total	22	9	31

Main Article

¹These authors contributed equally to this article.

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Page updated: August 25, 2021

Page reviewed: August 25, 2021

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