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Volume 28, Number 11—November 2022
Research Letter

Environmental Investigation during Legionellosis Outbreak, Montérégie, Quebec, Canada, 2021

Laura AtikesséComments to Author , Nabila Kadaoui, Vincent Lavallée, Éric Levac, Marie St-Amour, and François Milord
Author affiliations: Centre intégré de santé et de services sociaux de la Montérégie-Centre, Longueuil, Quebec, Canada (L. Atikessé, N. Kadaoui, V. Lavallée, É. Levac, M. St-Amour, F. Milord); Université de Sherbrooke, Longueuil (N. Kadaoui, É. Levac, M. St-Amour, F. Milord); Public Health Agency of Canada, Ottawa, Ontario, Canada (V. Lavallée)

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Abstract

In August 2021, a legionellosis outbreak involving 7 persons occurred within a 500-meter radius in the Montérégie region of Québec, Canada. Near real-time modeling of wind direction along with epidemiologic and environmental investigations identified the possible source. Modeling wind direction could help identify likely Legionella pneumophila sources during legionellosis outbreaks.

On August 11, 2021, a third reported legionellosis case in the Montérégie region, Quebec, Canada, triggered an outbreak investigation. Using published guidelines for legionellosis investigations (1,2), the investigation team sought to find the source of infection and rapidly stop the outbreak (3). However, confirmation of environmental sources of Legionella pneumophila, the bacteria that causes legionellosis, is not always possible (4).

The outbreak comprised 7 identified cases, 5 in men and 2 in women. Case-patient ages were 56–85 years, and all had a positive urinary antigen test for L. pneumophila serogroup 1. Patient symptoms began during July 29–August 18, 2021; thus, the incubation period was during July 19–August 16. Five case-patients lived within a 500-meter radius in the same neighborhood but were not otherwise acquainted. The other 2 case-patients visited that same area, 1 during July 28–29, the other on August 1.

Within a 3-km radius of the target area, 5 water cooling towers (numbers 1–5) were in operation in 3 facilities. We collected water samples from the 5 towers during August 12–13 and analyzed samples by PCR and culture (Appendix). We also reviewed results of periodic water sampling conducted on the towers during the previous 12 months; only 1 result was above normal, but it was below Quebec’s threshold of 1 million CFU/L for L. pneumophila, which requires owners to shut off ventilation, immediately decontaminate the system, and notify public health authorities. A sample collected on July 21 from cooling tower 1 was in the range of 10,000–100,000 CFU/L for L. pneumophila, triggering mandatory regulatory remediation actions, which the owner implemented. Nonetheless, we requested the owner of that cooling tower stop using the ventilator until we obtained further results.

After receiving PCR results for all cooling towers, we also requested a ventilator shutdown for cooling tower 5 on August 14 because of L. pneumophila detection. Subsequent information showed cooling tower 5 had no biocide during July 26–August 14; the tower was flushed and decontaminated on August 15 and 16, and we collected a control sample after decontamination. We reviewed control results for cooling towers 1 and 5, then informed the buildings’ managers they could restart the ventilators.

Using published protocols (5,6), the municipality and a private laboratory took water and swab samples from a rainwater retention pond located near cooling towers 1 and 2 and collected samples in 5 aeration ponds of a sewage treatment plant located near the other 3 cooling towers. All 6 ponds were equipped with aerating fountains, but swab samples were taken from the fountain only in the rainwater retention pond. Cultures identified L. pneumophila serogroup 2–15 in samples from 1 aerated pond at the sewage treatment plant, but no intervention was undertaken. Cultures from all other ponds were negative.

Only 2 respiratory specimens could be collected from the 7 hospitalized patients. One clinical specimen was L. pneumophila–positive by PCR, but cultures were negative, and sequence-based typing was inconclusive; thus, we could not match human and environmental isolates.

Figure

Wind rose profiles during each patient’s exposure period used in environmental investigation for legionellosis outbreak, Montérégie, Quebec, Canada, 2021. A–G) Cases C1–C7. Center crosshairs indicate center of the target area for each case; radii indicate percentage of frequency of winds over a time period, plotted by wind direction, with color bands showing wind speed ranges. Median and calm windspeeds are indicate for exposure times for each case. We calculated wind rose profiles for each of 7 case-patients during the time they were likely exposed. Wind rose profiles were generated by using meteorological data from High Resolution Deterministic Prediction System (HRDPS) modeling. Most case-patients, C1–C3 (panels A–C), C6 (panel G), and C7 (panel F), resided in the outbreak neighborhood; cases C4 (panel D) and C5 (panel E) were only in the area for a few hours, enabling more discriminating assessment of the possible exposures. C, case.

Figure. Wind rose profiles during each patient’s exposure period used in environmental investigation for legionellosis outbreak, Montérégie, Quebec, Canada, 2021. A–G) Cases C1–C7. Center crosshairs indicate center of the target area...

We collaborated with Environment and Climate Change Canada (ECCC) to identify potential L. pneumophila sources by examining meteorological conditions during exposure periods. ECCC created atmospheric dispersion models to illustrate wind directions during each case-patient’s exposure periods (Figure). Two case-patients were not residents of the area and only visited the community for a few hours, which enabled us to use the wind data as discriminatory support for our source hypothesis. ECCC’s model showed the most likely sources were cooling towers 1 and 2 and a nearby rainwater retention pond (Appendix Figures 1, 2). Using near real-time modeling, we triaged and prioritized investigation of exposure sources in the south and west because model findings illustrated little likelihood that exposure originated from the north or northeast (Figure). Modeling showed cooling tower 5 was a low probability source, but absence of biocides during the exposure period raises questions.

Another legionellosis outbreak investigation in Canada showed that few cases result from exposure within a 3- to 10-km radius of the L. pneumophila source (7). Nonetheless, we expanded our search to cooling towers within a 10-km radius of the target area (cooling towers 6–11) but found no contributing source (Appendix Figures 1, 2).

Our environmental investigation included supplementary information from many partners. Because outbreaks have been linked to misting equipment in grocery stores (8) and 5 of 7 case-patients shopped at the same grocery store, we took samples from the store’s water system and misting nozzles, but all cultures were negative for L. pneumophila. In addition, no tanker trucks were used for cleaning or reducing dust on the roads nor watering flowers in the affected area.

In conclusion, although none of the sampled cooling towers had microbiologic results above the sanitary threshold and we were not able to confirm the source by sequence-type matching, evidence suggests that cooling tower 1 was involved in this legionellosis outbreak. This investigation showed the usefulness of near real-time wind direction modeling, which could help identify likely L. pneumophila sources in future outbreaks.

Ms. Atikessé is an environmental health planning, programming and research officer at the Montérégie Public Health Department. Her primary research interest is environmental health.

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Acknowledgment

We acknowledge the valuable work of Alain Malo and Philippe Barnéoud, all the partner agencies involved (Régie du bâtiment du Québec, Centre d’expertise en analyse environnementale du Québec, Institut national de santé publique, Laboratoire de santé publique du Québec, Ministère des Transports du Québec, Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec, Environment et Climate Change Canada), and the Montérégie Public Health Department crew who participated in this outbreak investigation.

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References

  1. Ministère de la Santé et des Services sociaux du Québec. Intervention guide: legionellosis [in French]. Longueuil (Quebec, Canada): The Ministry; 2015 [cited 2021 Jan 5]. https://publications.msss.gouv.qc.ca/msss/fichiers/2015/15-271-03W.pdf
  2. US Centers for Disease Control and Prevention. Legionella (Legionnaires’ disease and Pontiac fever) things to consider: outbreak investigations [cited 2021 Jan 5]. https://www.cdc.gov/legionella/health-depts/epi-resources/outbreak-investigations.html
  3. Public Health England. Guidance on the control and prevention of Legionnaires’ disease in England: technical paper 1—disease surveillance, 2010 [cited 2021 Jan 5]. https://webarchive.nationalarchives.gov.uk/ukgwa/20140714084352/http://www.hpa.org.uk/webc/HPAwebFile/HPAweb_C/1279889007321
  4. Lock  K, Millett  C, Heathcock  R, Joseph  CA, Harrison  TG, Lee  JV, et al.; Outbreak Control Team. Public health and economic costs of investigating a suspected outbreak of Legionnaires’ disease. Epidemiol Infect. 2008;136:130614. DOIPubMedGoogle Scholar
  5. US Centers for Disease Control and Prevention. Sampling procedure and potential sampling sites. Atlanta (GA): The Centers; 2019 [cited 2021 Jan 5]. https://www.cdc.gov/legionella/downloads/cdc-sampling-procedure.pdf
  6. Ministère de la Santé et des Services sociaux du Québec. Technical sheet of the intervention guide – Legionellosis. Operational information for the management of a case or outbreak potentially associated with a spa [in French]. Longueuil (Quebec, Canada): The Ministry; 2021.
  7. Goupil-Sormany I, Huot C. Legionellosis outbreak in Quebec City, Quebec, Canada, summer 2012 [in French]. Quebec: Capitale-Nationale Health and Social Services Agency; 2012 [cited 2021 Jan 5]. https://numerique.banq.qc.ca/patrimoine/details/52327/2242741
  8. Barrabeig  I, Rovira  A, Garcia  M, Oliva  JM, Vilamala  A, Ferrer  MD, et al. Outbreak of Legionnaires’ disease associated with a supermarket mist machine. Epidemiol Infect. 2010;138:18238. DOIPubMedGoogle Scholar

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Cite This Article

DOI: 10.3201/eid2811.220151

Original Publication Date: October 17, 2022

Table of Contents – Volume 28, Number 11—November 2022

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Laura Atikessé, Centre intégré de santé et de services sociaux de la Montérégie-Centre, 1255 rue Beauregard, Longueuil, QC J4K 2M3, Canada

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Page created: October 06, 2022
Page updated: October 24, 2022
Page reviewed: October 24, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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