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Volume 28, Number 12—December 2022
Dispatch

Monkeypox Virus Detection in Different Clinical Specimen Types

Maan Hasso1, Stephen Perusini1, Alireza Eshaghi, Elaine Tang, Romy Olsha, Hanyue Zhang, Evelyn Lau, Ashleigh Sullivan, Kirby Cronin, Seyeon Lee, Janet Obando, Cedric DeLima, Sandeep Nagra, Tom Braukmann, Venkata R. Duvvuri, Melissa Richard-Greenblatt, Antoine Corbeil, Julianne V. Kus, Anna Majury, Samir Patel, and Jonathan B. GubbayComments to Author 
Author affiliations: Public Health Ontario, Toronto, Ontario, Canada (M. Hasso, S. Perusini, A. Eshaghi, E. Tang, R. Olsha, H. Zhang, E. Lau, A. Sullivan, K. Cronin, S. Lee, J. Obando, C. DeLima, S. Nagra, T. Braukmann, V.R. Duvvuri, M. Richard-Greenblatt, A. Corbeil, J.V Kus, A. Majury, S. Patel, J.B. Gubbay); University of Toronto, Toronto (V.R. Duvvuri, M. Richard-Greenblatt, A. Corbeil, J.V. Kus, S. Patel, J.B. Gubbay); University of Oxford, Oxford, UK (M. Richard-Greenblatt); Queen's University, Kingston, Ontario, Canada (A. Majury); The Hospital for Sick Children, Toronto (J.B. Gubbay)

Main Article

Table

Detection results for MPXV by real-time reverse transcription PCR in clinical specimen types submitted to the Public Health Ontario Laboratory, Toronto, Ontario, Canada*

Specimen types and number Blood, n = 190 Nasal/NP, 
n = 137 Throat/OP, 
n = 106 Skin lesions, 
n = 559 Urine, n = 41
Positive
 29 (15.3)
 20 (14.6)
 31 (29.2)
 177 (31.7)
 6/41 (14.6)
Specimens from positive patients
 29/67 (43.3)
 20/36 (55.6)
 31/46 (67.4)
 177/213 (83.1)
 6/21 (28.6)
Target
Orthopoxvirus
No. positive 26 16 24 135 5
Mean Ct (SD), range 38.6 (4.1), 27.2‒40.0 36.3 (6.3), 23.3‒38.8 32.0 (6.5), 17.5‒38.0 27.1 (7.3), 14.3‒ 39.6 37.3 (5.1), 29.0‒37.9
Monkeypox
No. positive 15 11 13 74 4
Mean Ct (SD), range 35.9 (2.1), 32.2‒37.9 32.4 (5.6), 18.2‒37.7 27.8 (5.1), 19.2‒36.0 23.1 (6.5), 12.0‒37.9 32.2 (5.5), 27.2‒37.7
Clade II
No. positive 15 13 13 75 4
Mean Ct (SD), range 35.3 (2.3), 31.2‒37.1 32.8 (5), 18.1‒37.6 27.3 (4.5), 19.6‒35.2 23.1 (6.6), 11.1‒37.4 32.2 (5.1), 27.7‒ 37.2

*Values are no. (%) except as indicated. Among 30 additional specimens not shown, MPXV was detected in 2/5 semen, 1/4 saliva, 0/1 cerebrospinal fluid, and none of 20 specimens with undocumented sources. Indeterminate results are not included; those are blood 6/190 (3.2%), nasal/NP 5/137 (3.6%), throat/OP 2/106 (1.9%), skin lesion 10/559 (1.8%), and urine 6/41 (14.6%). Ct, cycle threshold; MPXV, monkeypox virus; NP, nasopharyngeal; OP, oropharyngeal.

Main Article

1These authors contributed equally to this article.

Page created: October 01, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
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