Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 28, Number 2—February 2022
Research

Role of Anopheles Mosquitoes in Cache Valley Virus Lineage Displacement, New York, USA

Constentin Dieme1Comments to Author , Kiet A. Ngo1, Shaun Tyler, Joseph G. Maffei, Steven D. Zink, Alan P. Dupuis, Cheri A. Koetzner, Chelsea Shultis, Jessica Stout, Anne F. Payne, P. Bryon Backenson, Lili Kuo, Michael A. Drebot, Alexander T. Ciota, and Laura D. Kramer
Author affiliations: New York State Department of Health, Slingerlands, New York, USA (C. Dieme, K.A. Ngo, J.G. Maffei, S.D. Zink, A.P. Dupuis, C.A. Koetzner, C. Shultis, J. Stout, A.F. Payne, L. Kuo, A.T. Ciota, L.D. Kramer); Public Health Agency of Canada, Winnipeg, Manitoba, Canada (S. Tyler, M.A Drebot); New York State Department of Health, Albany, New York, USA (P.B. Backenson); State University of New York at Albany, Albany (A.T. Ciota, L.D. Kramer)

Main Article

Table 6

Infection, dissemination, and transmission rates for Anopheles quadrimaculatus mosquitoes for different Cache Valley virus isolates, New York, USA, 2000‒2016*

Strain Blood meal titer, log10 PFU/mL Day postinfection No. infected/no. tested (%) No. disseminated/no. tested (%) No. transmitted/no. tested (%)
L1-NY10 7.1 2 25/25 (100) 18/25 (72)† NT
6 42/42 (100) 42/42 (100) 5/42 (11.90)
15
 41/41 (100)
 41/41 (100)
 5/41 (12.20)
L1-NY25 6.6 2 18/25 (72)‡ 3/18 (16.67)† NT
6 31/35 (88.57)‡ 16/31 (51.61)† 0/16 (0)
15
 32/35 (91.43)‡
 23/32 (71.88)†
 4/23 (17.39)
L2–15350152 7.2 2 25/25 (100) 25/25 (100) NT
6 37/37 (100) 37/37 (100) 11/37 (29.73)
15
 44/44 (100)
 44/44 (100)
 24/44 (54.54)
L2–15330577 7.1 2 25/25 (100) 25/25 (100) NT
6 35/35 (100) 35/35 (100) 7/35 (20)
15
 35/35 (100)
 35/35 (100)
 10/35 (28.57)
L2-Hu2011 6.3 2 25/25 (100) 25/25 (100) NT
6 30/30 (100) 30/30 (100) 8/30 (26.67)
15 30/30 (100) 30/30 (100) 9/30 (30)
5.5 2 25/25 (100) 25/25 (100) NT
6 30/30 (100) 30/30 (100) 1/30 (3.33)
15
 24/24 (100)
 24/24 (100)
 5/24 (20.83)
R-15041084 7.1 2 25/25 (100) 25/25 (100) NT
6 41/41 (100) 41/41 (100) 6/41 (14.63)
15
 34/34 (100)
 34/34 (100)
 18/34 (52.94)
R-PA 5 2 10/25 (40) 4/10 (40) NT
6 21/35 (60) 3/21 (14.29) 0/3 (0)
15 30/35 (85.71) 0/30 (0) NT
R-15041170 7 2 25/25 (100) 25/25 (100) NT
6 30/30 (100) 30/30 (100) 1/30 (3.33)
15 30/30 (100) 30/30 (100) 11/30 (36.67)
4.7 2 24/25 (96) 11/24 (45.83) NT
6 30/30 (100) 29/30 (96.67) 0/29 (0)
15 25/27 (92.59) 23/25 (92) 1/23 (4.34)
R-15060131 7.4 2 25/25 (100) 25/25 (100) NT
6 30/30 (100) 30/30 (100) 2/30 (6.67)
15 21/21 (100) 21/21 (100) 4/21 (19.05)
4.1 2 25/25 (100) 17/25 (68) NT
6 30/30 (100) 28/30 (93.33) 1/28 (3.57)
15 29/30 (96.67) 26/29 (89.66) 2/26 (7.69)

*L1, lineage 1; L2, lineage 2; NT, not tested; R, reassortant (containing lineage 1 large RNA segment and lineage 2 small and medium RNA segments); NT, not tested. †p<0.05: lineage 1 strains dissemination rate compared with lineage 2 strains when mosquito fed on infectious blood ≈6.3–7.4 log10 PFU/mL titer. ‡p<0.05: lineage 1 strain infection rate compared with lineage 2 strains when mosquito fed on infectious blood ≈6.3–7.4 log10 PFU/mL titer.

Main Article

1These authors contributed equally to this article.

Page created: October 17, 2021
Page updated: January 22, 2022
Page reviewed: January 22, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external