Volume 28, Number 3—March 2022
Dispatch
Transovarial Transmission of Heartland Virus by Invasive Asian Longhorned Ticks under Laboratory Conditions
Wilson R. Raney, Josiah B. Perry, and Meghan E. Hermance
Table 1
Rate of detection of HRTV RNA by qRT-PCR and infectious HRTV by FFA in adult Haemaphysalis longicornis ticks at 14, 21, 28, and 40 dpi*
| Procedure | Real-time qRT-PCR detection of HRTV RNA, no. positive/no. tested (%) |
FFA titration of HRTV, whole tick | ||
|---|---|---|---|---|
| Salivary glands | Midgut | Carcass | ||
| Medium injected | 0/15 (0) | 0/15 (0) | 0/15 (0) | 0/9 (0) |
| HRTV-injected 14 dpi | 4/8 (50) | 8/8 (100) | 8/8 (100) | 5/5 (100) |
| HRTV-injected 21 dpi | 7/8 (88) | 8/8 (100) | 8/8 (100) | 5/5 (100) |
| HRTV-injected 28 dpi | 6/8 (75) | 8/8 (100) | 8/8 (100) | 5/5 (100) |
| HRTV-injected 40 dpi | 3/6 (50) | 6/6 (100) | 6/6 (100) | 4/5 (80) |
*dpi, days postinjection; FFA, focus-forming assay; HRTV, Heartland virus; qRT-PCR, quantitative reverse transcription PCR.
Page created: December 08, 2021
Page updated: February 21, 2022
Page reviewed: February 21, 2022
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