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Volume 28, Number 3—March 2022

Transovarial Transmission of Heartland Virus by Invasive Asian Longhorned Ticks under Laboratory Conditions

Wilson R. Raney, Josiah B. Perry, and Meghan E. HermanceComments to Author 
Author affiliation: University of South Alabama College of Medicine, Mobile, Alabama, USA

Main Article

Table 2

Rate of detection of HRTV RNA by qRT-PCR and infectious HRTV by FFA in fed Haemaphysalis longicornis adult tick carcasses, tick eggs, and tick larvae*

Procedure Real-time qRT-PCR detection of HRTV RNA, no. positive/no. tested (%)
FFA titration of HRTV, larvae pools‡
Fed adult carcasses Egg pools† Larvae pools†
Medium injected 0/5 (0) 0/15 (0) 0/20 (0) 0/5 (0)
HRTV-injected 5/5 (100) 15/15 (100) 20/20 (100) 5/5 (100)

*FFA, focus-forming assay; HRTV, Heartland virus; qRT-PCR, quantitative reverse transcription PCR. † Egg and larvae pools contained 50 eggs or larvae/pool. ‡Larvae pools contained 150 larvae/pool.

Main Article

Page created: December 08, 2021
Page updated: February 21, 2022
Page reviewed: February 21, 2022
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