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Volume 28, Number 3—March 2022
Synopsis

Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta

Sarah Reagan-Steiner1Comments to Author , Julu Bhatnagar1, Roosecelis B. Martines, Nicholas S. Milligan, Carly Gisondo, Frank B. Williams, Elizabeth Lee, Lindsey Estetter, Hannah Bullock, Cynthia S. Goldsmith, Pamela Fair, Julie Hand, Gillian Richardson, Kate R. Woodworth, Titilope Oduyebo, Romeo R. Galang, Rebecca Phillips, Elizaveta Belyaeva, Xiao-Ming Yin, Dana Meaney-Delman, Timothy M. Uyeki, Drucilla J. Roberts, and Sherif R. Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (S. Reagan-Steiner, J. Bhatnagar, R.B. Martines, E. Lee, L. Estetter, H. Bullock, C.S. Goldsmith, P. Fair, K.R. Woodworth, T. Oduyebo, R.R. Galang, D. Meaney-Delman, T.M. Uyeki, S.R. Zaki); Tulane University School of Medicine, New Orleans, Louisiana, USA (N.S. Milligan, E. Belyaeva, X.-M. Yin); Oschner Health, New Orleans (C. Gisondo, F.B. Williams, R. Phillips); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (E. Lee); Synergy America, Inc., Duluth, Georgia, USA (L. Estetter, H. Bullock); Louisiana Department of Health, Baton Rouge, Louisiana, USA (J. Hand, G. Richardson); Massachusetts General Hospital, Boston, Massachusetts, USA (D.J. Roberts)

Main Article

Figure 2

In situ hybridization (ISH) slides demonstrating localization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomic RNA in heart, liver, and lymph node tissues and electron microscopic evidence of viral particles in heart tissue from neonate in the United States that died with SARS-CoV-2 infection and placental histopathology and angiotensin-converting enzyme-2 immunohistochemical stain slides. A) SARS-CoV-2 RNA staining by nucleocapsid gene ISH assay in the endothelial cells in myocardium vessel walls (arrow). Original magnification ×20. B) Extracellular virus particles in the connective tissue of the heart (arrow). Scale bar indicates 100 nm. C) Intravascular staining by nucleocapsid gene ISH assay in the liver parenchyma (arrow). Original magnification ×20. D) Extensive nucleocapsid gene ISH staining within macrophages of subcapsular sinus of lymphoid follicle in the submucosa of upper airway (arrow). Original magnification ×10. E) Second trimester placenta with fibrinoid necrosis (arrow). Original magnification ×20. F) Angiotensin-converting enzyme 2 immunostaining in the membrane polarized on the maternal lake side in the syncytiotrophoblast (arrow). Original magnification ×63.

Figure 2. In situ hybridization (ISH) slides demonstrating localization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomic RNA in heart, liver, and lymph node tissues and electron microscopic evidence of viral particles in heart tissue from neonate in the United States that died with SARS-CoV-2 infection and placental histopathology and angiotensin-converting enzyme-2 immunohistochemical stain slides. A) SARS-CoV-2 RNA staining by nucleocapsid gene ISH assay in the endothelial cells in myocardium vessel walls (arrow). Original magnification ×20. B) Extracellular virus particles in the connective tissue of the heart (arrow). Scale bar indicates 100 nm. C) Intravascular staining by nucleocapsid gene ISH assay in the liver parenchyma (arrow). Original magnification ×20. D) Extensive nucleocapsid gene ISH staining within macrophages of subcapsular sinus of lymphoid follicle in the submucosa of upper airway (arrow). Original magnification ×10. E) Second trimester placenta with fibrinoid necrosis (arrow). Original magnification ×20. F) Angiotensin-converting enzyme 2 immunostaining in the membrane polarized on the maternal lake side in the syncytiotrophoblast (arrow). Original magnification ×63.

Main Article

1These authors contributed equally to this article.

Page created: December 02, 2021
Page updated: February 21, 2022
Page reviewed: February 21, 2022
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