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Volume 28, Number 3—March 2022
Research

Evaluation of Commercially Available High-Throughput SARS-CoV-2 Serologic Assays for Serosurveillance and Related Applications

Mars Stone1Comments to Author , Eduard Grebe1, Hasan Sulaeman, Clara Di Germanio, Honey Dave, Kathleen Kelly, Brad J. Biggerstaff, Bridgit O. Crews, Nam Tran, Keith R. Jerome, Thomas N. Denny, Boris Hogema, Mark Destree, Jefferson M. Jones, Natalie Thornburg, Graham Simmons, Mel Krajden, Steve Kleinman, Larry J. Dumont, and Michael P. Busch
Author affiliations: Vitalant Research Institute, San Francisco, California, USA (M. Stone, E. Grebe, H. Sulaeman, C. Di Germanio, H. Dave, K. Kelly, G. Simmons, L.J. Dumont, M.P. Busch); University of California–San Francisco, San Francisco (M. Stone, E. Grebe, G. Simmons, M.P. Busch); South African Centre for Epidemiological Modelling and Analysis, Stellenbosch University, Stellenbosch, South Africa (E. Grebe); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (B.J. Biggerstaff); University of California Irvine Medical Center, Orange, California, USA (B.O. Crews); University of California–Davis, Davis, California, USA (N. Tran); Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington, USA (K.R. Jerome); Duke Human Vaccine Institute, Duke University, Durham, North Carolina, USA (T.N. Denny); Sanquin Research, Amsterdam, the Netherlands (B. Hogema); BloodWorks NorthWest, Seattle (M. Destree); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J.M. Jones, N. Thornburg); British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada (M. Krajden); University of British Columbia, Vancouver (S. Kleinman); University of Colorado School of Medicine, Denver, Colorado, USA (L.J. Dumont)

Main Article

Figure 5

Intraclass correlation coefficients based on blinded replicate sample testing, reflecting the proportion of total variance that is between-sample rather than within-sample variability of severe acute respiratory syndrome coronavirus 2 antibody detection in study of commercially available high-throughput assays for serosurveillance. Results falling outside the primary measurement range were excluded. On-board dilutions were used to estimate reactivity in specimens where initial results fell outside the primary measurement range. Horizontal dotted lines show conventional (although arbitrary) thresholds for moderate (0.5), good (0.75), and excellent (0.9) repeatability (17). Assays are described in Table 1. Ab, antibody; Ag, antigen; N, nucleocapsid; PRNT, plaque reduction neutralization test; RBD, receptor binding domain; S, spike protein.

Figure 5. Intraclass correlation coefficients based on blinded replicate sample testing, reflecting the proportion of total variance that is between-sample rather than within-sample variability of severe acute respiratory syndrome coronavirus 2 antibody detection in study of commercially available high-throughput assays for serosurveillance. Results falling outside the primary measurement range were excluded. On-board dilutions were used to estimate reactivity in specimens where initial results fell outside the primary measurement range. Horizontal dotted lines show conventional (although arbitrary) thresholds for moderate (0.5), good (0.75), and excellent (0.9) repeatability (17). Assays are described in Table 1. Ab, antibody; Ag, antigen; N, nucleocapsid; PRNT, plaque reduction neutralization test; RBD, receptor binding domain; S, spike protein.

Main Article

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Main Article

1These first authors contributed equally to this article.

Page created: January 12, 2022
Page updated: February 21, 2022
Page reviewed: February 21, 2022
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