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Volume 28, Number 4—April 2022
Research Letter

Pseudo-Outbreak of Bordetella parapertussis Caused by Contaminated Swabs in the Netherlands

Jacky FlipseComments to Author , Angelino T. Tromp, Janneke Bosman, Christine ten Hove, Hans Beks, Titia Kortbeek, Guido J.H. Bastiaens, and Ellen M. Mascini
Author affiliations: Rijnstate, Arnhem, the Netherlands (J. Flipse, A.T. Tromp, J. Bosman, C. ten Hove, G.J.H. Bastiaens, E.M. Mascini); Safety and Public Health Services Gelderland Midden, Arnhem (H. Beks); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (T. Kortbeek)

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Abstract

An increase in positive Bordetella parapertussis tests among patients in a teaching hospital in the Netherlands resulted in enhanced infection control and microbiological surveillance. Further analysis revealed that batches of contaminated nasopharyngeal swabs were associated with a pseudo-outbreak, resulting in incorrect diagnoses, antimicrobial treatments, isolation precautions, and public health notifications.

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Epidemiologic timeline of Bordetella parapertussis–positive findings in a pseudo-outbreak caused by contaminated swabs, the Netherlands, 2020–2021. PCR results positive and negative for B. parapertussis are denoted for pediatric patients, their family members, healthcare workers, and other care providers.

Figure. Epidemiologic timeline of Bordetella parapertussis–positive findings in a pseudo-outbreak caused by contaminated swabs, the Netherlands, 2020–2021. PCR results positive and negative for B. parapertussisare denoted for...

We report a pseudo-outbreak of Bordetella parapertussis in the Department of Pediatrics in Rijnstate, an 809-bed teaching hospital in the Netherlands. The department provides level II care to infants, neonates, and preterm infants. In March 2021, we diagnosed B. parapertussis in 3 infants hospitalized for respiratory symptoms by using an in-house PCR against insertion sequences (IS) IS481 and IS1001 (1). During calendar week 21 (Figure), we identified more B. parapertussis cases in the same department, bringing the total case count to 5 in neonates, 1 in a toddler, and 6 in infants. Several of these patients were born prematurely.

PCR-positive case-patients had pertussis-like complaints, and we confirmed B. parapertussis in the patients or their siblings. We traced all positive tests to the Department of Pediatrics. Because we suspected nosocomial transmission, we started contact tracing investigations among parents and healthcare workers (HCWs) and identified B. parapertussis in another 4 patients and in 3 HCWs.

Cases among HCWs were particularly unexpected. Because of the coronavirus disease (COVID-19) pandemic, all HCW were using type IIR surgical masks and keeping >1.5 m distance from each other. In addition, all patients had private rooms, and we observed no increase in other respiratory pathogens.

Because we discovered additional B. parapertussis–positive cases, we upgraded HCW masks to FFP1, the recommended type for pertussis (2). We also confirmed instructions regarding continued HCW social distancing, including during lunch breaks, to prevent further B. parapertussis spread. We then implemented extended screening for asymptomatic cases among all HCWs and relatives of B. parapertussis–positive case-patients. Among 22 HCWs tested, 72% (16/22) tested IS1001-positive.

Parallel to actions in the clinic, we checked the possibility of laboratory contamination. The laboratory uses several controls to confirm sensitivity and specificity of assays; all controls consistently showed correct results. Swipe-tests did not reveal contaminated surfaces or equipment. All cases had relatively high cycle threshold (Ct) values (median Ct 35), as seen with prior B. parapertussis results from the laboratory (n = 17). In addition, 2 other laboratories confirmed B. parapertussis in original clinical samples and DNA eluates by targeting diverse regions of the IS1001 gene using an in-house PCR (1) or Real Accurate Quadruplex Bordetella PCR (PathoFinder, https://www.pathofinder.com). Confirmatory PCR tests also had high Ct values.

Finally, we tested unused ESwab 483CE nasopharyngeal swabs (Copan, https://www.copanusa.com), which included a flocked swab and 1 mL of liquid Amies medium in a plastic, screw cap tube. Liquid Amies media from 7 batches (1 sample per batch) and flocked tips from 2 batches (2 tips per batch) were available for testing. All liquid Amies media were PCR-negative, but both batches of flocked swab tips were PCR-positive for IS1001. Moreover, 2 flocked tips were placed in 0.5 mL of Milli-Q water (Millipore, https://www.emdmillipore.com), a 4-fold higher concentration than for standard diagnostic tests. In the higher concentration, we saw lower Ct values (Ct ≈35) compared with regular diagnostic tests (Ct >37).

We retested all 23 PCR-positive HCWs by using individually packaged 503CS01 flocked swabs (Copan) from a PCR-negative batch; 22 HCWs tested PCR-negative and 1 tested PCR-positive. Upon re-examination, we found that testing for the positive HCW case was not performed with an individually packaged swab provided by the laboratory but an ESwab from the suspect batch. Although unintentional, this case proved that the B. parapertussis could be traced to IS1001-positive nasopharyngeal swabs tips. No B. parapertussis could be cultured, which aligns with the notion that the swabs are gamma-irradiated after packaging. Gamma irradiation kills bacteria but does not affect DNA.

We alerted clinical and molecular microbiologists in the Netherlands, the supplier, and the Health Inspectorate regarding swabs contaminated with B. parapertussis IS1001-containing DNA. Subsequently, >6 laboratories in the Netherlands recognized and reported false-positive B. parapertussis to the National Institute of Public Health and the Environment. Contamination appeared to be associated with specific ESwab batch numbers (Appendix), which explains why the pseudo-outbreak focused on 1 department in our hospital. The contamination was confirmed by the manufacturer, but the source was not disclosed.

B. parapertussis can cause pertussis-like symptoms, although symptoms usually are milder and occur less frequently than with B. pertussis (3). Each year, ≈6,400 B. pertussis cases, are notified in the Netherlands based on culture, PCR, or serology, but only 26 B. parapertussis cases are notified (Appendix Figure). During 2020–2021, the COVID-19 pandemic and associated social distancing measures caused a large decrease in reported B. pertussis cases. Of note, B. parapertussis reports did not diminish during this period (Appendix), possibly because testing strategies changed, contaminated swabs were already circulating, or both.

Our report illustrates the importance of critically evaluating of microbiological results lacking clinical and epidemiologic clues. We were confronted with a growing number of neonatal patients and HCWs with unexplained B. parapertussis–positive tests. Because these tests are requested infrequently, it took months before contamination with IS1001-like DNA in nasopharyngeal swabs became clear. Clinicians and public health agencies should be aware of the possibility of false-positive microbiology results and consider contaminated products when unexplainable results are found.

Dr. Flipse is a medical molecular microbiologist at the laboratory for Medical Microbiology and Immunology at Rijnstate Hospital. His primary interests are on molecular diagnostics and medical microbiology.

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Acknowledgment

We thank our colleagues in the laboratory, the Department of Pediatrics, Infection Prevention, and the Safety and Public Health Service Gelderland Midden for their contributions to this work. We thank Canisius Wilhelmina Hospital (Nijmegen, the Netherlands) and the University Medical Center Utrecht (Utrecht, the Netherlands) for assistance in confirming contamination on the swabs and the Working Group on Molecular Diagnostics of Infectious Diseases for the national survey on contaminated swabs. We also thank Dimphey van Meijeren for collecting the data on the notifications to the Center for Infectious Disease Control.

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References

  1. Antila  M, He  Q, de Jong  C, Aarts  I, Verbakel  H, Bruisten  S, et al. Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. J Med Microbiol. 2006;55:104351. DOIPubMedGoogle Scholar
  2. Siegel  JD, Rhinehart  E, Jackson  M, Chiarello  L; Health Care Infection Control Practices Advisory Committee. 2007 Guideline for isolation precautions: preventing transmission of infectious agents in health care settings. Am J Infect Control. 2007;35(Suppl 2):S65164. DOIPubMedGoogle Scholar
  3. Plotkin  SA, Orenstein  W, Offit  P, Edwards  KM. Plotkin’s vaccines. 7th ed. Philadelphia: Elsevier; 2017.

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Figure

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Cite This Article

DOI: 10.3201/eid2804.212097

Original Publication Date: March 09, 2022

Table of Contents – Volume 28, Number 4—April 2022

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Jacky Flipse, Ziekenhuis Rijnstate Arnhem, Laboratory for Medical Microbiology and Immunology, President Kennedylaan 100, Velp 6883AZ, the Netherlands

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Page created: February 27, 2022
Page updated: March 19, 2022
Page reviewed: March 19, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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