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Volume 28, Number 4—April 2022
Research Letter

Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA

Adriana HeguyComments to Author , Dacia Dimartino, Christian Marier, Paul Zappile, Emily Guzman, Ralf Duerr, Guiqing Wang, Jonathan Plitnick, Alexis Russell, Daryl M. Lamson, and Kirsten St. George
Author affiliations: NYU Langone Health, New York, New York, USA (A. Heguy, D. Dimartino, C. Marier, P. Zappile, E. Guzman); NYU Grossman School of Medicine, New York (A. Heguy, R. Duerr, G. Wang); New York State Department of Health, Albany, New York, USA (J. Plitnick, A. Russell, D.M. Lamson, K. St. George); State University of New York at Albany, Albany (K. St. George)

Main Article

Table

Results of repeated library preparations and sequencing of 25 random SARS-CoV-2 samples with Omicron variant and mutation previously assigned as P681R, including an alternative chemistry and sequencing platform*

Location
 NYULH Genome Technology Center
 NYSDOH
Protocol xGen SARS-CoV-2 Amp Panel 96rxn† AmpliSeq SARS-CoV-2 Insight‡
Reverse transcriptase SuperScript IV Maxima H Minus SuperScript VILO cDNA synthesis kit§
PCR cycles, no. P681R calls
18, 0 18, 0 27, 0
24, 1
 24, 1 NA

*NA, not applicable; NYSDOH, New York State Department of Health, Albany, NY, USA, which performed ThermoFisher sequencing chemistry on 12 samples; NYULH, NYU Langone Health, New York, NY, USA, which performed Illumina sequencing chemistry on 13 different samples; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. †Integrated DNA Technologies (https://eu.idtdna.com). ‡ThermoFisher Scientific (https://www.thermofisher.com). §Invitrogen (https://www.thermofisher.com).

Main Article

Page created: January 26, 2022
Page updated: March 19, 2022
Page reviewed: March 19, 2022
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