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Volume 28, Number 6—June 2022
Research

Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso

Andrew G. Letizia1, Catherine B. Pratt1Comments to Author , Michael R. Wiley, Anne T. Fox, Mba Mosore, Bright Agbodzi, Clara Yeboah, Selassie Kumordjie, Nicholas Di Paola, Kone Cisse Assana, David Coulidiaty, Casimir Ouedraogo, Joseph H. Kofi Bonney, William Ampofo, Zékiba Tarnagda, and Lassana SangaréComments to Author 
Author affiliations: Naval Medical Research Unit TWO, Singapore (A.G. Letizia); University of Nebraska Medical Center, Omaha, Nebraska, USA (C.B. Pratt, M.R. Wiley); Naval Medical Research Unit THREE, Ghana Detachment, Accra, Ghana (A.T. Fox); Noguchi Memorial Institute for Medical Research, Accra (M. Mosore, B. Agbodzi, C. Yeboah, S. Kumordjie, J.H.K. Bonney, W. Ampofo); US Army Medical Research Institute of Infectious Disease, Frederick, Maryland, USA (N. Di Paola); Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso (K.C. Assana, Z. Tarnagda); Centre Hospitalier Universitaire Yalgado Ouédraogo, Ouagadougou, Burkina Faso (D. Coulidiaty, C. Ouedraogo, L. Sangaré)

Main Article

Table

Suspected dengue virus–positive samples from the 2017 Burkina Faso dengue virus outbreak, found to be positive by CDC Trioplex real-time RT-PCR or serologic testing, and sequencing results for samples that generated genomes with >85% coverage*

NMIMR laboratory ID Specimen collection date, 2017 PCR results, Ct Sequencing results
Serologic results
Serotype Genome coverage, % GenBank accession no.
NS1 Ag IgM IgG
IP-002 Oct 16 UND + DENV-2 99.6 MT261956
IP-008 Oct 16 35.5 + DENV-3 99.1 MT261972
IP-009 Oct 16 37.1 + DENV-2 99.7 MT261957
IP-012 Oct 16 40.6 + DENV-2 87.6 MT261958
IP-029 Oct 17 37.5 + DENV-2 98.8 MT261959
IP-036 Oct 17 40.6 + DENV-2 99.5 MT261960
IP-091 Oct 26 36 + DENV-2 99.8 MT261961
IP-099 Oct 25 40.3 + DENV-2 99.6 MT261962
IP-103 Oct 25 34.6 + DENV-2 99.7 MT261963
IP-112 Oct 24 39.5 + DENV-2 99.7 MT261964
IP-121 Oct 23 29 + DENV-1 99.7 MT261951
IP-127 Oct 23 38.2 DENV-2 99.4 MT261965
IP-153 Nov 8 33.7 + DENV-2 99.8 MT261966
IP-159 Nov 9 32 + DENV-1 99.3 MT261952
IP-171 Nov 9 33.8 + + + DENV-3 94.3 MT261973
IP-179 Nov 13 22.5 + DENV-3 94.4 MT261974
IP-194 Nov 17 25.2 DENV-3 99.7 MT261975
IP-226 Oct 4 25.4 DENV-3 99.8 MT261976
IP-242 Oct 9 29.5 DENV-1 99.6 MT261953
IP-246 Oct 9 31.1 DENV-2 99.8 MT261967
IP-267 Oct 12 19.3 DENV-3 99.8 MT261977
IP-270 Oct 12 37.1 DENV-2 99.5 MT261968
IP-304 Oct 27 24.1 + DENV-3 99.7 MT261978
IP-307 Oct 30 37.3 DENV-2 99.7 MT261969
IP-310 Nov 2 30.3 + + DENV-3 95.7 MT261979
IP-314 Nov 2 23.8 + DENV-1 99.7 MT261954
IP 387 Dec 12 UND + DENV-1 88.4 MT261955
IP 494 Nov 3 41.2 + DENV-2 99.6 MT261970
IP 666 Nov 6 UND + DENV-2 99.6 MT261971

*CDC, US Centers for Disease Control Prevention; Ct, cycle threshold; NMIMR, Noguchi Memorial Institute for Medical Research; NS1 Ag, nonstructural protein 1 antigen; RT-PCR, reverse transcription PCR; UND, undetected; +, positive; –, negative.

Main Article

1These first authors contributed equally to this article.

Page created: March 18, 2022
Page updated: May 22, 2022
Page reviewed: May 22, 2022
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