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Volume 28, Number 6—June 2022
Research Letter

Detecting SARS-CoV-2 Omicron B.1.1.529 Variant in Wastewater Samples by Using Nanopore Sequencing

Lasse D. RasmussenComments to Author , Stine R. Richter, Sofie E. Midgley, and Kristina T. Franck
Author affiliation: Author affiliation: Statens Serum Institut, Copenhagen, Denmark

Main Article

Table

Results of read mapping and consensus sequence analysis for detection of SARS-CoV-2 Omicron B.1.1.529 variant in 2 wastewater treatment plants, Denmark*

Sequencing
Time, hours
Wastewater treatment plant 1

Wastewater treatment plant 2
Mapped reads
Omicron mapping consensus mutations
Mapped reads
Omicron mapping consensus
mutations
Delta
Omicron
Delta
Omicron
1 17122 783 T23104A, C23202A, C23525T, C23604A 21743 1292 C23202A, C23525T, C23604A
2 30980 1446 T23075C, C23202A, C23525T, C23604A 40040 2326 C22971T, T23075C, C23202A, C23525T, C23604A
3 41320 1967 T23075C, C23202A, C23525T, C23604A 58254 3453 G22992C, C22995A, T23075C, C23202A, C23525T, C23604A

*Boldface indicates Omicron-specific mutations. Mutation A23403G was omitted because it is present in Delta and Omicron, but it was present in all consensus sequences.

Main Article

Page created: March 30, 2022
Page updated: May 22, 2022
Page reviewed: May 22, 2022
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