Volume 28, Number 7—July 2022
Highly Pathogenic Avian Influenza A(H5N8) Clade 184.108.40.206b Virus in Dust Samples from Poultry Farms, France, 2021
Avian influenza A(H5N8) virus has caused major epizootics in Europe since 2016. We conducted virologic analysis of aerosol and dust collected on poultry farms in France during 2020–2021. Our results suggest dust contributes to viral dispersal, even early in an outbreak, and could be a valuable surveillance tool.
Avian influenza is a viral disease caused by influenza A viruses, segmented, negative, single-stranded RNA viruses belonging to the Orthomyxoviridae family. Wild aquatic birds are the virus reservoir and generate occasional worldwide panzootic outbreaks during seasonal migrations (1). Highly pathogenic avian influenza (HPAI) virus subtypes can cause panzootic outbreaks associated with high mortality in wild and domestic birds, as well as substantial economic losses for the poultry industry, and are a major threat to public health because of their zoonotic potential.
During winter 2020–21, the HPAI H5N8 virus belonging to the A/goose/Guangdong/1/1996 clade 220.127.116.11b lineage caused hundreds of outbreaks among wild and domestic flocks across Europe (2,3). France was severely affected; 492 poultry farms, primarily duck farms, were infected during December 5, 2020–May 3, 2021. Despite reinforced surveillance activities, the virus spread rapidly, posing major challenges for surveillance and control. Officially recognized surveillance methods involve tracheal or cloacal swab-based sampling (4,5). However, these methods are laborious and have technical requirements that make application on such a massive scale difficult; thus, newer surveillance methods are needed.
Epidemiologic modeling of this outbreak suggested within-farm viral transmission was extremely fast, and the environment was a major source of contamination for neighboring farms (6). HPAI viruses disperse in aerosols, in fomites carried by human and animal vectors, and via feathers, fecal particles, and to a great extent, dust (7–9). Poultry farms are known to heavily generate dust particles that spread from feed, litter, feces, and animal skin and feathers (9,10). These particles can act as vehicles for bacteria and viruses and are classified, depending on their size, as inhalable (<100 µm), thoracic (<10 µm), or respirable (<4 µm) (10). In poultry houses, most dust consists of nonrespirable particles >4 µm (10). We evaluated the role of dust as a vehicle of H5N8 clade 18.104.22.168b virus and assessed whether dust or aerosol sampling is a viable alternative to bird swab sampling for HPAI virus surveillance.
During December 2020–April 2021, we conducted a study in 63 poultry houses located in 4 departments (administrative units) in France highly affected by HPAI H5N8 virus outbreaks. On the basis of daily official outbreak reports, we identified HPAI-infected poultry houses and poultry houses in close vicinity or with epidemiologic links to infected houses. The study included a total of 48 duck houses, 12 chicken houses, 2 quail houses, and 1 goose house. We selected farms identified as being near an HPAI outbreak to reflect a range of sanitary statuses and infection stages (i.e., no, mild, or severe clinical signs; high mortality rates). We specifically included houses without clinical signs among animals to evaluate virus dispersal and dust testing for HPAI surveillance in the early stages of infection.
In each selected poultry house, we collected surface dust with 2 wipes on the building’s walls and feeders (9,11) (Appendix). In 19 houses, we also collected aerosol samples by using 2 devices, Coriolis Compact (Bertin Instruments, https://www.bertin-instruments.com) and the NIOSH BC 251 developed by the National Institute for Occupational Safety and Health (NIOSH; https://www.cdc.gov/niosh) (Appendix). Furthermore, we collected tracheal swab samples from 20 randomly selected birds in each house (Appendix Table 1). We chose tracheal over cloacal swab samples because the typical respiratory shedding and tropism of HPAI H5N8 clade 22.214.171.124 viruses enables earlier detection in the respiratory tract than cloacae (12,13).
We performed real-time quantitative reverse transcription PCR on all samples to detect HPAI virus at the molecular level by targeting the matrix protein and H5 genes (Appendix). We compared cycle threshold (Ct) distributions of each sample by using raincloud plots and a boxplot model (Figure 1). In general, Ct values for tracheal swabs (≈25.2) and dust (≈28.6) were similar (Figure 1; Appendix). Between the 2 aerosol collectors, the Coriolis device showed more positive results (Ct <40) than the NIOSH BC 251 sampler. Furthermore, we noted HPAI H5N8 virus was more easily detected in the largest particles, those >1 µm (Figure 1). These results suggest that the HPAI H5N8 virus dispersion is associated with large dust particles, which could be a major vehicle for viral spread.
To estimate the sensitivity of the 4 different sampling methods (tracheal swab samples, surface wipes, and Coriolis and NIOSH aerosol samplers) in houses with or without poultry showing clinical signs, we used a latent class modeling approach, necessary when no standard has been established (14). We adjusted the model to cross-detect each farm by the 4 different sampling methods and estimated model parameters in a Bayesian framework (Appendix). Model outputs suggested that the different sampling methods had equivalent sensitivity in HPAI-infected flocks showing clinical signs. Surface dust and aerosol sampling showed substantially higher sensitivity in HPAI-infected flocks without clinical signs, but the difference was not statistically significant despite overlap of 95% credible intervals (Table 1; Figure 2).
Finally, to assess the infectiousness of environmental samples, we processed 25 surface dust or aerosol samples taken from 5 animal houses and used these for virus isolation in embryonated eggs (Appendix). Among 25 samples, 12 (48%) tested positive, confirming that viral isolation is possible from these sampling methods (Table 2).
We used field conditions to evaluate whether dust from poultry farms contained HPAI viruses and to compare surface dust and aerosol testing for HPAI virus against official swab-based methods. We used wipe tests to collect surface dust and 2 bioaerosol devices to collect aerosol samples during the 2020–21 HPAI H5N8 virus epizootic outbreak in France. Standard molecular analysis detected high viral RNA loads in the early phase of flock infection, before clinical signs appeared. In addition, size fractioning of aerosol samples revealed that high RNA viral loads and infectious viral particles were associated with the largest particles (>1 µm), which are easy to collect and use for molecular analysis. However, the field conditions we used cannot be reproduced in experimental animal trials because of ethical and biosecurity requirements, which result in dramatically lower dust loads than those found in the field.
Recent research on influenza transmission routes revealed that nonrespiratory airborne particles are more likely to cause infection than are droplets or fomites (7). Infectious aerosols generated from inert objects handled by humans or dispersed through animal movements can lead to further infection. Dust can carry infectious particles and is omnipresent in poultry houses (10) and so could be a major means of viral transmission and dispersal in the environment. These findings suggest that biosecurity protocols should strongly emphasize limiting the amount of dust dispersed via farm equipment to reduce the spread of HPAI viruses.
Of note, for early detection, before flock animals show clinical signs of illness, we found that surface dust sampling using wipe tests and aerosol sampling using a high flow rate collection device are more sensitive than tracheal swab samples. The higher sensitivity of environmental sampling methods for early detection is likely because of infection dynamics at the flock level. During the early phases of infection, only a few animals are infectious, making the probability of detecting virus during individual swab-based sampling low (6). Swab sampling also is time consuming, labor-intensive, and expensive, whereas dust wiping is inexpensive, fast, easy to perform, and noninvasive.
In conclusion, we detected HPAI H5N8 clade 126.96.36.199b virus in dust samples from poultry farms during a large epizootic in France. Our findings suggest dust wipe samples are an efficient surveillance tool and could enable more rapid virus detection and implementation of measures to curb virus spread.
Mr. Filaire is an engineer in biosciences and a PhD candidate in a collaborative project between the Host-Pathogen Interactions Joint Research Unit, National Veterinary School and INRAe, Toulouse, France; EIP Purpan, Toulouse; and Theseo, Lanxess Group, Laval, France. His research focus is on innovative methods for detection and characterization of emerging viruses.
We thank Bertin Instruments, France, and the National Institute for Occupational Safety & Health (NIOSH), United States, for the loan of aerosol collectors.
This study was performed in the framework of the Chair for Avian Biosecurity, hosted by the National Veterinary College of Toulouse and funded by the Direction Générale de l’Alimentation, Ministère de l’Agriculture et de l’Alimentation, France. F.F. is funded by Theseo, a company of the LanXess Group, France. This work also received financial support from the FEDER/Région Occitanie Recherche et Sociétés 2018-AI-TRACK.
- Munster VJ, Baas C, Lexmond P, Waldenström J, Wallensten A, Fransson T, et al. Spatial, temporal, and species variation in prevalence of influenza A viruses in wild migratory birds. PLoS Pathog. 2007;3:
- Lewis NS, Banyard AC, Whittard E, Karibayev T, Al Kafagi T, Chvala I, et al. Emergence and spread of novel H5N8, H5N5 and H5N1 clade 188.8.131.52 highly pathogenic avian influenza in 2020. Emerg Microbes Infect. 2021;10:148–51.
- Adlhoch C, Fusaro A, Kuiken T, Niqueux É, Staubach C, Terregino C, et al.; European Food Safety Authority; European Centre for Disease Prevention and Control and European Union Reference Laboratory for Avian Influenza. Avian influenza overview May - August 2020. EFSA J. 2020;18:
- World Organisation for Animal Health (OIE). Avian influenza (including infection with high pathogenicity avian influenza viruses). In OIE terrestrial manual 2021. Geneva: The Organisation; 2021.
- Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Depner K, Drewe JA, et al.; EFSA Panel on Animal Health and Welfare (EFSA AHAW Panel). (EFSA AHAW Panel). Scientific opinion on the assessment of the control measures of the category A diseases of Animal Health Law: Highly Pathogenic Avian Influenza. EFSA J. 2021;19:
- Vergne T, Gubbins S, Guinat C, Bauzile B, Delpont M, Chakraborty D, et al. Inferring within-flock transmission dynamics of highly pathogenic avian influenza H5N8 virus in France, 2020. Transbound Emerg Dis. 2021;68:3151–5.
- Asadi S, Gaaloul Ben Hnia N, Barre RS, Wexler AS, Ristenpart WD, Bouvier NM. Influenza A virus is transmissible via aerosolized fomites. Nat Commun. 2020;11:4062.
- Spekreijse D, Bouma A, Koch G, Stegeman A. Quantification of dust-borne transmission of highly pathogenic avian influenza virus between chickens. Influenza Other Respir Viruses. 2013;7:132–8.
- Lopez KM, Nezworski J, Rendahl A, Culhane M, Flores-Figueroa C, Muñoz-Aguayo J, et al. Environmental sampling survey of H5N2 highly pathogenic avian influenza–infected layer chicken farms in Minnesota and Iowa. Avian Dis. 2018;62:373–80.
- Zhao Y, Aarnink AJA, De Jong MCM, Groot Koerkamp PWG. Airborne microorganisms from livestock production systems and their relation to dust. Crit Rev Environ Sci Technol. 2014;44:1071–128.
- Carrique-Mas JJ, Breslin M, Sayers AR, McLaren I, Arnold M, Davies R. Comparison of environmental sampling methods for detecting Salmonella in commercial laying flocks in the UK. Lett Appl Microbiol. 2008;47:514–9.
- Gaide N, Foret-Lucas C, Figueroa T, Vergne T, Lucas MN, Robertet L, et al. Viral tropism and detection of clade 184.108.40.206b H5N8 highly pathogenic avian influenza viruses in feathers of ducks and geese. Sci Rep. 2021;11:5928.
- Beerens N, Germeraad EA, Venema S, Verheij E, Pritz-Verschuren SBE, Gonzales JL. Comparative pathogenicity and environmental transmission of recent highly pathogenic avian influenza H5 viruses. Emerg Microbes Infect. 2021;10:97–108.
- van Smeden M, Naaktgeboren CA, Reitsma JB, Moons KG, de Groot JA. Latent class models in diagnostic studies when there is no reference standard—a systematic review. Am J Epidemiol. 2014;179:423–31.
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Original Publication Date: May 31, 2022