Figure 2. Phylogenetic tree of hemagglutinin genes of genetic group 2 (G2) of highly pathogenic avian influenza A(H5N1/H5N8) viruses isolated on the Izumi Plain, Japan, in November 2021. We phylogenetically analyzed the nucleotide sequences of the genes from A/environment/Kagoshima/KU-ngrB4/2021 (mixed), A/chicken/Kagoshima/21A6T/2021 (H5N1), A/chicken/Kagoshima/B3T/2021 (H5N8), and A/hooded crane/Kagoshima/KU-5T/2021 (H5N8) with representative counterparts by using the maximum-likelihood method with a bootstrapping set of 1,000 replicates. Bold text indicates viruses isolated in this study. Bootstrap values >70% are shown at the nodes. Scale bar indicates the number of nucleotide substitutions per site. HPAI, highly pathogenic avian influenza; LPAI, low pathogenicity avian influenza.