Volume 28, Number 8—August 2022
Dispatch
Child Melioidosis Deaths Caused by Burkholderia pseudomallei–Contaminated Borehole Water, Vietnam, 2019
Table 2
Culture results and genotype data from environmental samples in a study of 3 child melioidosis deaths caused by Burkholderia pseudomallei–contaminated borehole water, Vietnam, 2019*
Sample type, date | No. samples | No. sampling points† | Qualitative culture |
Median quantitative count, CFU (range) | No. isolates selected for MLST‡ | ST | |
---|---|---|---|---|---|---|---|
No. (%) positive samples | No. (%) positive sampling points† | ||||||
Sampling 1, 2019 Nov 17 | |||||||
Front garden soil | 7 | 7 | 0 | 0 | NP | NA | |
Water from borehole A | 3 | 1 | 3 (100) | 1 (100) | 2§ | 3 | 541 |
Water from borehole B | 3 | 1 | 0 | 0 | NP | NA | NA |
Water from borehole C | 3 | 1 | 0 | 0 | NP | NA | NA |
Boiled drinking water |
1 |
1 |
0 |
0 |
NP |
NA |
NA |
Sampling 2, 2019 Nov 23 | |||||||
Back garden soil near borehole A | 46 | 10 | 27 (58.7) | 8 (80) | 406 (12–746)§ | 6 | 541 |
Rice field soil | 40 | 8 | 5 (12.5) | 2 (25) | ND | 5 | 541 |
Water from borehole A | 26 | 1 | 26 (100) | 1 (100) | ND | 4 | 541 |
Water from borehole B | 3 | 1 | 0 | 0 | NP | NA | NA |
Water from borehole C | 3 | 1 | 0 | 0 | NP | NA | NA |
Water from neighbors’ borehole | 33 | 11 | 0 | 0 | NP | NA | NA |
Water from ponds | 30 | 10 | 0 | 0 | NP | NA | NA |
*CFU, colony forming unit; MLST, multilocus sequence typing; NA, not applicable; ND, not detected; NP, not performed; ST, sequence type. †Sampling points refer to garden, borehole, field, and pond sites. ‡We selected 20 B. pseudomallei isolates for sampling; 2 patient isolates are not shown here. §B. pseudomallei colonies were countable only in 2 borehole water samples and 5 garden soil samples (Appendix). In water samples CFU/mL; in soil samples CFU/g.