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Volume 28, Number 9—September 2022
Research

Detection of Endosymbiont Candidatus Midichloria mitochondrii and Tickborne Pathogens in Humans Exposed to Tick Bites, Italy

Giovanni Sgroi1, Roberta Iatta1, Piero Lovreglio, Angela Stufano, Younes Laidoudi, Jairo Alfonso Mendoza-Roldan, Marcos Antonio Bezerra-Santos, Vincenzo Veneziano, Francesco Di Gennaro, Annalisa Saracino, Maria Chironna, Claudio Bandi, and Domenico OtrantoComments to Author 
Author affiliations: University of Bari Aldo Moro, Bari, Italy (G. Sgroi, R. Iatta, P. Lovreglio, A. Stufano, Y. Laidoudi, J.A. Mendoza-Roldan, M.A. Bezerra-Santos, F. Di Gennaro, A. Saracino, M. Chironna, D. Otranto); University of Naples Federico II, Naples, Italy (V. Veneziano); Osservatorio Faunistico Venatorio, Naples (V. Veneziano); University of Milan Statale, Milan, Italy (C. Bandi); Bu-Ali Sina University, Hamedan, Iran (D. Otranto)

Main Article

Table 1

Bacteria investigated by molecular methods in a study on the relationship between endosymbiont Candidatus Midichloria mitochondrii and tickborne pathogens in humans exposed to tick bites, Italy, 2021

Tickborne bacteria Target gene Primers Sequence, 5ˈ → 3ˈ Base pairs Reference
Coxiella burnetii IS1111a Trans1 TATGTATCCACCGTAGCCAGT 687 (21)
Trans2
 CCCAACAACACCTCCTTATTC
Rickettsia spp. gltA CS 78F GCAAGTATCGGTGAGGATGTAAT 401 (22)
CS 323R
 GCTTCCTTAAAATTCAATAAATCAGGAT
Borrelia burgdorferi sensu lato complex Flagellin FLA1 AGAGCAACTTACAGACGAAATTAAT 482 (23)
FLA2
 CAAGTCTATTTTGGAAAGCACCTAA
Anaplasmataceae 16S rRNA EHR 16SD GGTACCYACAGAAGAAGTCC 345 (24)
EHR 16SR TAGCACTCATCGTTTACAGC

Main Article

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1These authors contributed equally to this article.

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