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Volume 28, Number 9—September 2022
Research

Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection

Dominik Nörz, Hui Ting Tang, Petra Emmerich, Katja Giersch, Nicole Fischer, Stephan Schmiedel, Marylyn M. Addo, Martin Aepfelbacher, Susanne Pfefferle, and Marc LütgehetmannComments to Author 
Author affiliations: University Medical Center Hamburg-Eppendorf, Hamburg, Germany (D. Nörz, H.T. Tang, K. Giersch, N. Fischer, S. Schmiedel, M.M. Addo, M. Aepelbacher, S. Pfefferle, M. Lütgehetmann); Bernhard-Nocht Institute for Tropical Medicine, Hamburg (P. Emmerich, M.M. Addo, S. Pfefferle); German Center for Infection Research, Hamburg (M.M. Addo)

Main Article

Figure 2

Amplification curves of clinical samples, including internal controls for dual-target monkeypox virus assay rapidly adapted from established high-throughput molecular testing infrastructure. A) Nonvariola orthopoxvirus; B) monkeypox virus; C) internal control. Samples included clinical swab specimens of monkeypox lesions, oropharyngeal swab samples, and EDTA plasma from patients with confirmed monkeypox, Hamburg, Germany. Asterisk (*) in panel B indicates the positive control curve in channel 2, which was the cell culture supernatant of Congo Basin monkeypox strain collected in 1987. West Africa strain samples exhibit a reduction of approximately one third in relative fluorescence increase for monkeypox virus, due to a known mismatch in the probe region (Appendix Figure 1).

Figure 2. Amplification curves of clinical samples, including internal controls for dual-target monkeypox virus assay rapidly adapted from established high-throughput molecular testing infrastructure. A) Nonvariola orthopoxvirus; B) monkeypox virus; C) internal control. Samples included clinical swab specimens of monkeypox lesions, oropharyngeal swab samples, and EDTA plasma from patients with confirmed monkeypox, Hamburg, Germany. Asterisk (*) in panel B indicates the positive control curve in channel 2, which was the cell culture supernatant of Congo Basin monkeypox strain collected in 1987. West Africa strain samples exhibit a reduction of approximately one third in relative fluorescence increase for monkeypox virus, due to a known mismatch in the probe region (Appendix Figure 1).

Main Article

Page created: July 13, 2022
Page updated: August 19, 2022
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