Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 29, Number 1—January 2023
Research

Molecular Tools for Early Detection of Invasive Malaria Vector Anopheles stephensi Mosquitoes

Om P. SinghComments to Author , Taranjeet Kaur, Gunjan Sharma, Madhavinadha P. Kona, Shobhna Mishra, Neera Kapoor, and Prashant K. Mallick
Author affiliations: National Institute of Malaria Research, New Delhi, India (O.P. Singh, T. Kaur, G. Sharma, M.P. Kona, S. Mishra, P.K. Mallick); Indira Gandhi National Open University, New Delhi (N. Kapoor)

Main Article

Table 2

Results of hydrolysis real-time PCR and size-diagnostic PCR on individual and pooled mosquitoes for early detection of invasive malaria vector Anopheles stephensi mosquitoes*

Specimen type DNA isolation method Real-time PCR
Size-diagnostic PCR
No. Ct values (An. stephensi)† Ct values IC No. An. stephensi
specific band
IC band
Anophelines
Neocellia series
An. stephensi type DNeasy 26 13.47–17.23 15.20–18.93 24 Positive Positive
An. stephensi intermediate DNeasy 2 14.75–15.60 15.39–16.06 2 Positive Positive
An. stephensi var. mysorensis DNeasy 1 14.54 15.01 1 Positive Positive
An. stephensi strain STE2 DNeasy 1 14.85–15.25 16.06–16.51 2 Positive Positive
An. stephensi type form Boiling 10 15.17–17.41 15.97–18.05 - Not done Not done
An. stephensi type form, single leg DNeasy 4 18.64–22.29 19.02–23.51 4 Positive Positive
Pyretophorus series
An. gambiae DNeasy 3 Negative 14.58–17.32 3 Negative Positive
An. quadrimaculatus DNeasy 2 Negative 14.52–15.64 2 Negative Positive
An. merus DNeasy 2 Negative 15.22–15.35 2 Negative Positive
An. subpictus form A DNeasy 2 Negative 16.61–17.02 1 Negative Positive
An. subpictus form A‡ Pre-isolated§ 1 33.09 18.32 Negative Positive
An. subpictus form B Pre-isolated§ 2 Negative 16.17–16.35 1 Negative Positive
An. sundaicus cytoform D Pre-isolated§ 1 Negative 14.47 1 Negative Positive
Albimanus series
An. albimanus DNeasy 2 Negative 16.21–16.58 1 Negative Positive
Anopheles series
An. freeborni DNeasy 2 Negative 15.73–15.81 1 Negative Positive
An. atroparvus DNeasy 2 Negative 14.70–15.88 1 Negative Positive
Neomyzomyia series
An. dirus DNeasy 2 Negative 17.34–18.01 1 Negative Positive
An. farauti DNeasy 2 Negative 19.09–19.48 1 Negative Positive
Myzomyia series
An. funestus DNeasy 2 Negative 15.50–15.62 1 Negative Positive
An. culicifacies species A DNeasy 1 Negative 17.41 1 Negative Positive
An. culicifacies species B Pre-isolated§ 1 Negative 17.48 1 Negative Positive
An. fluviatilis species S Pre-isolated§ 1 Negative 16.61 1 Negative Positive
An. fluviatilis species T
Pre-isolated§
1
Negative
17.02

1
Negative
Positive
Culicines
Culex quinquefasciatus DNeasy 3 Negative 16.58–19.20 2 Negative Positive
Aedes albopictus DNeasy 2 Negative 16.65–16.68 2 Negative Positive
Ae. aegypti DNeasy 2 Negative 18.96–19.20 2 Negative Positive
Ae. aegypti
Pre-isolated§
1
32.95
20.97


Negative
Positive
Single An. stephensi individual pooled with An. culicifacies
Adults (1/2) DNeasy 1 17.06 18.31 1 Positive Positive
Adults (1/5) DNeasy 1 15.37 16.11 1 Positive Positive
Adults (1/10) DNeasy 1 15.77 15.30 1 Positive Positive
Adults (1/20) DNeasy 1 15.74 13.71 1 Positive Positive
Adults (1/50) DNeasy 1 15.43 11.43 1 Positive Positive
Adults (1/25) DNAzol 1 14.53 12.61 1 Positive Positive
Adults (1/100) DNAzol 1 16.37 11.27 1 Positive Positive
Adults (1/500) DNAzol 2 20.12–23.96 11.08–11.50 2 Positive Positive
Larvae (1/2) DNeasy 1 15.56 16.87 1 Positive Positive
Larvae (1/5) DNeasy 1 15.49 16.65 1 Positive Positive
Larvae (1/10) DNeasy 1 16.36 15.53 1 Positive Positive
Larvae (1/20) DNeasy 1 16.51 15.06 1 Positive Positive
Larvae (1/50) DNeasy 1 16.27 14.40 1 Positive Positive
Larvae (1/100)
DNAzol
1
18.14
13.16

1
Positive
Positive
Single An. stephensi individual pooled with field collected mosquitoes (mixed mosquito-species)¶
Adults (1/100) DNAzol 1 17.61 11.70 1 Positive Positive

*Ct, cycle threshold; IC, internal control. †Ct value shown as Negative implies that no Ct value was scored within 35 cycles of reactions. ‡Contaminated with An. stephensi DNA. §Preserved DNA isolated manually for other studies. ¶Denominator of numeric expression inside parenthesis indicate total number of mosquitoes in a pool.

Main Article

Page created: November 10, 2022
Page updated: December 21, 2022
Page reviewed: December 21, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external