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Volume 29, Number 3—March 2023
Dispatch

Extended Viral Shedding of MERS-CoV Clade B Virus in Llamas Compared with African Clade C Strain

Jordi Rodon, Anna Z. Mykytyn, Nigeer Te1, Nisreen M.A. Okba, Mart M. Lamers, Lola Pailler-García, Guillermo Cantero, Irina Albulescu, Berend-Jan Bosch, Malik Peiris, Albert Bensaid, Júlia Vergara-Alert, Bart L. HaagmansComments to Author , and Joaquim SegalésComments to Author 
Author affiliations: Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Spain (J. Rodon, N. Te, L. Pailler-García, G. Cantero, A. Bensaid, J. Vergara-Alert, J. Segalés); IRTA, Programa de Sanitat Animal, CReSA, Campus de la Universitat Autònoma de Barcelona, Bellaterra (J. Rodon, N. Te, L. Pailler-García, G. Cantero, A. Bensaid, J. Vergara-Alert); Erasmus Medical Centre, Rotterdam, the Netherlands (A.Z. Mykytyn, N.M.A. Okba, M.M. Lamers, B.L. Haagmans); Utrecht University, Utrecht, the Netherlands (I. Albulescu, B.-J. Bosch); The University of Hong Kong, Hong Kong, China (M. Peiris); Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra (J. Segalés)

Main Article

Figure 2

Mixed model analyzing the transmission competence of each MERS-CoV strain over time in investigation of llamas experimentally infected with MERS-CoV. Boxplots show daily virus shedding of sentinel llamas infected with MERS-CoV Egypt/2013 (red) or Qatar15/2015 strains (blue) after direct exposure to inoculated llamas. A, B) Genomic (A) and subgenomic (B) viral RNA quantification in nasal swabs collected throughout the study. C) Infectious MERS-CoV titers. Only the time points considered in the mixed models are represented. Horizontal lines within boxes indicate medians; box tops and bottoms indicate interquartile ranges; error bars indicate 95% CIs; black dots indicate outliers. p values are indicated above the boxes; p values indicate statistical differences between areas under the curve of the experimental groups, as calculated by Wilcoxon test. Cq, quantification cycle; MERS-CoV, Middle East respiratory syndrome coronavirus; TCID50, 50% tissue culture infective dose.

Figure 2. Mixed model analyzing the transmission competence of each MERS-CoV strain over time in investigation of llamas experimentally infected with MERS-CoV. Boxplots show daily virus shedding of sentinel llamas infected with MERS-CoV Egypt/2013 (red) or Qatar15/2015 strains (blue) after direct exposure to inoculated llamas. A, B) Genomic (A) and subgenomic (B) viral RNA quantification in nasal swabs collected throughout the study. C) Infectious MERS-CoV titers. Only the time points considered in the mixed models are represented. Horizontal lines within boxes indicate medians; box tops and bottoms indicate interquartile ranges; error bars indicate 95% CIs; black dots indicate outliers. p values are indicated above the boxes; p values indicate statistical differences between areas under the curve of the experimental groups, as calculated by Wilcoxon test. Cq, quantification cycle; MERS-CoV, Middle East respiratory syndrome coronavirus; TCID50, 50% tissue culture infective dose.

Main Article

1Current affiliation: The University of Hong Kong, Hong Kong, China

Page created: January 04, 2023
Page updated: February 20, 2023
Page reviewed: February 20, 2023
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