Volume 7, Number 1—February 2001
Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
|PCR amplification procedure||No. of samples (%)||1||2||1 & 2||3|
|COWP gene fragment amplified|
|COWP gene fragment not amplifiedb|
|Oocysts detected by microscopy||35 (2.1)|
|Microscopy not reconfirmedc||2 (0.1)|
|Oocysts not reconfirmed by microscopy||43 (2.6)|
aAll samples previously negative by unnested procedure.
bBy both unnested and nested procedures.
cInsufficient material available.