TY - JOUR AU - Pedraza-Díaz, Susana AU - Amar, Corinne AU - Nichols, Gordon L. AU - McLauchlin, Jim T1 - Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene T2 - Emerging Infectious Disease journal PY - 2001 VL - 7 IS - 1 SP - 49 SN - 1080-6059 AB - We developed a sensitive nested polymerase chain reaction (PCR) procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases. KW - United Kingdom DO - 10.3201/eid0701.700049 UR - https://wwwnc.cdc.gov/eid/article/7/1/70-0049_article ER - End of Reference