TY - JOUR AU - Ebel, Gregory AU - Dupuis, Alan AU - Ngo, Kiet AU - Nicholas, David AU - Kauffman, Elizabeth AU - Jones, Susan AU - Young, Donna AU - Maffei, Joseph AU - Shi, Pei-Yong AU - Bernard, Kristen AU - Kramer, Laura T1 - Partial Genetic Characterization of West Nile Virus Strains, New York State, 2000 T2 - Emerging Infectious Disease journal PY - 2001 VL - 7 IS - 4 SP - 650 SN - 1080-6059 AB - We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999. KW - West Nile Virus KW - WNV KW - Flavivirus KW - Evolution KW - Envelope Gene KW - United States DO - 10.3201/eid0704.017408 UR - https://wwwnc.cdc.gov/eid/article/7/4/01-7408_article ER - End of Reference