TY - JOUR AU - Andreadis, Theodore AU - Anderson, John AU - Vossbrinck, Charles T1 - Mosquito Surveillance for West Nile Virus in Connecticut, 2000: Isolation from Culex pipiens, Cx. restuans, Cx. salinarius, and Culiseta melanura T2 - Emerging Infectious Disease journal PY - 2001 VL - 7 IS - 4 SP - 670 SN - 1080-6059 AB - Fourteen isolations of West Nile (WN) virus were obtained from four mosquito species (Culex pipiens [5], Cx. restuans [4], Cx. salinarius [2], and Culiseta melanura [3]) in statewide surveillance conducted from June through October 2000. Most isolates were obtained from mosquitoes collected in densely populated residential locales in Fairfield and New Haven counties, where the highest rates of dead crow sightings were reported and where WN virus was detected in 1999. Minimum field infection rates per 1,000 mosquitoes ranged from 0.5 to 1.8 (county based) and from 1.3 to 76.9 (site specific). Cx. restuans appears to be important in initiating WN virus transmission among birds in early summer; Cx. pipiens appears to play a greater role in amplifying virus later in the season. Cs. melanura could be important in the circulation of WN virus among birds in sylvan environments; Cx. salinarius is a suspected vector of WN virus to humans and horses. KW - West Nile virus KW - mosquitoes KW - isolation KW - identification KW - Vero cell culture KW - RT-PCR KW - Culex pipiens KW - Culex restuans KW - Culex salinarius KW - Culiseta melanuray KW - United States DO - 10.3201/eid0704.017413 UR - https://wwwnc.cdc.gov/eid/article/7/4/01-7413_article ER - End of Reference