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Volume 7, Number 4—August 2001
West Nile Virus
West Nile Virus

Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay

Donna J. JohnsonComments to Author , Eileen N. Ostlund, Douglas D. Pedersen, and Beverly J. Schmitt
Author affiliations: Animal and Plant Health Inspection Service, U. S. Department of Agriculture, Ames, Iowa, USA

Main Article


Virus isolation compared with plaque reduction virus neutralization, immunoglobulin M-capture enzyme-linked immunosorbent assay serologic results, or both for West Nile virus samples positive by reverse transcription-nested polymerase chain reaction

RT-nPCR positive samples No. tested VI positive VI negative Seropositive Seronegative
Equine brain 13 10 3 13 0
Equine plasma 8a 1 7 0 8b
Avian brain 7 7 0 NAc NA

RT-nPCR = reverse transcription-nested polymerase chain reaction; VI = virus isolation; NA = not applicable.
aMultiple plasma samples collected from two experimentally challenged ponies were RT-nPCR-positive for West Nile virus between 3 and 7 days after inoculation.
bWest Nile virus antibodies were not detected in either pony until beyond the time the virus was detected by RT-nPCR.
cNo avian serum was available for testing.

Main Article

Page created: April 27, 2012
Page updated: April 27, 2012
Page reviewed: April 27, 2012
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