Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

Comparative West Nile Virus Detection in Organs of Naturally Infected American Crows (Corvus brachyrhynchos)

Nicholas A. Panella*Comments to Author , Amy J. Kerst*, Robert S. Lanciotti*, Patricia Bryant†, Bruce Wolf†, and Nicholas Komar*
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; †New Jersey Department of Health and Senior Services Virology Program, Trenton, New Jersey, USA

Main Article

Table

Amount of virus detected by Vero plaque assay and TaqMan reverse transcriptase polymerase chain reaction in American crow organs

Crow number Heart Kidney Liver Lung Spleen Brain
NJN 5 +++a/3.40E+03b +++/5.90E+04 ++ /8.48E+04 +++/2.42E+04 +++/4.09E+03 +++/5.41E+03
NJN 6 ++/2.36E+03 ++/1.12E+04 -c/1.10E+02 -/4.61E+02 +/2.02E+02 ++/3.12E+02
NJN 7 ++/9.57E+03 ++/5.52E+03 -/7.61E+01 +/3.46E+03 +/1.26E+02 ++/1.76E+03
NJN 8 +++/2.31E+05 +++/5.41E+04 +++/5.61E+05 +++/3.20E+04 +++/4.08E+04 +++/6.18E+04
NJN 9 +++/2.94E+04 +++/1.96E+05 +/2.15E+05 +++/4.36E+05 +++/1.17E+05 +++/1.78E+05
NJN 11 +++/3.62E+04 +++/1.06E+04 +++/1.06E+03 +++/1.31E+04 +++/1.07E+03 +++/2.15E+04
NJN 13 NT/NT +/2.54E+02 -/1.50E+00 -/1.68E+02 -/- +/9.28E+01
NJN 29 -/- -/- -/- -/- -/- ++/6.67E+00
NJN 30 -/- -/- -/- -/- -/- -/-
NJN 33 -/- -/- -/- -/- -/- -/ 3.24E+00
NJN 37 -/- -/- -/- -/- -/- -/ 2.10E-01
NJN 40 -/- -/- -/- -/- -/- -/-
NJN 41 -/- -/- -/- -/- -/- -/-
NJN 43 -/- -/- -/- -/- -/- -/-
NJN 44 -/- -/- -/- -/- -/- -/-
NJN 51 -/- -/- -/- -/- -/- -/-
NJN 57 -/- -/- -/- -/- -/- -/-
NJN 62 -/- -/- -/- -/- -/- -/-
NJN 75 -/- -/- -/- -/- -/- -/-
NJN 95 -/- -/- -/- -/- -/- -/-

aVero cell plaque assay: +++ = >100 PFU/200 µL, ++ = 10-100 PFU/200 µL, + = <10 PFU/200 µL.
bTaqMan RT-PCR assay: PFU equivalents/5 µL.
cNegative.
NT = Not tested.

Main Article

Page created: April 27, 2012
Page updated: April 27, 2012
Page reviewed: April 27, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external