Volume 8, Number 1—January 2002
Prevalence and Genetic Profiling of Virulence Determinants of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Beef, and Humans, Calcutta, India
|Primer no.||Nucleotide sequence of primers||Target||PCR conditionsa
|EVT1/ EVT2||5'-CAACACTGGATGATCTCAG-3' 5'-CCCCCTCAACTGCTAATA-3'||stx1 family||94°C, 60s||55°C, 60sb||72°C, 60s||349||20|
|EVS1/ EVC2||5'-ATCAGTCGTCACTCACTGGT-3' 5'-CTGCTGTCACAGTGACAAA-3'||stx2 family||94°C, 60s||55°C, 60sb||72°C, 60s||110||20|
|hlyA1/ hlyA4||5'-GGTGCAGCAGAAAAAGTTGTAG-3' 5'-TCTCGCCTGATAGTGTTTGGTA-3'||EHEC hlyA||94°C, 30s||57°C, 60s||72°C, 90s||1,551||7|
|wkat-B/ wkat-F||5'-CTTCCTGTTCTGATTCTTCTGG-3' 5'-AACTTATTTCTCGCATCATCC-3'||katP||94°C, 30s||56°C, 60s||72°C, 150s||2,125||9|
|D1/ D13R||5'-CGTCAGGAGGATGTTCAG-3' 5'-CGACTGCACCTGTTCCTGATTA-3'||etpD||94°C, 30s||52°C, 60s||72°C, 70s||1,062||21|
|EAE1/ EAE2||5'-AAACAGGTGAAACTGTTGCC-3' 5'-CTCTGCAGATTAACCTCTGC-3'||eae||94°C, 60s||55°C, 90s||72°C, 90s||350||22|
a Unless stated, PCR was done for 30 cycles.
b After 35 cycles, final extension step of 10 min at 72°C was performed.
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