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Volume 8, Number 10—October 2002
Letter

Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis Supplement

Main Article

Table 3

Results of real-time PCR and culture testing performed on 382 clinical specimensa,b


PCR only
PCR and culture
Total
Nine inhalational cases + - PCR + C - PCR + C + PCR - C - PCR - C +
Blood specimens
5
20
9
5
35
0
74
Swab specimens
0
0
0
0
0
0
0
Serum specimens
2
15
3
0
16
0
36
Sputum specimens
0
1
1
0
0
0
2
Tissue specimens
0
1
3
0
3
0
7
Pleural fluid







specimens
5
0
11
0
3
0
19
Other specimens
1
0
2
0
1
0
4
Totals
50
92
142




Seven cutaneous cases







Blood specimens
0
0
1
0
10
0
11
Swab specimens
0
0
0
0
3
0
3
Serum specimens
0
2
0
0
11
0
13
Sputum specimens
0
0
0
0
0
0
0
Tissue specimens
0
2
2
0
4
0
8
Other specimens
0
0
0
0
2
0
2
Totals
4
33
37




Four suspect cases







Blood specimens
0
2
0
0
7
0
9
Swab specimens
0
0
0
0
2
0
2
Serum specimens
0
1
0
0
3
0
4
Sputum specimens
0
0
0
0
0
0
0
Tissue specimens
0
2
0
0
0
0
2
 Other specimens
0
0
0
0
0
0
0
Totals
5
12
17




Other







Blood specimens
0
16
0
0
58
0
74
Swab specimens
0
1
0
0
14
0
15
Serum specimens
0
11
0
0
30
0
41
Sputum specimens
0
0
0
0
4
0
4
Tissue specimens
0
14
0
0
24
0
38
Other specimens 0 2 0 0 12 0 14
Totals 44 142 186

aSixteen patients with laboratory-confirmed anthrax, four suspect cases of anthrax, and 74 patients on whom anthrax has been ruled out.
bPCR, polymerase chain reaction; C, culture.

Main Article

Page created: July 19, 2010
Page updated: July 19, 2010
Page reviewed: July 19, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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