Volume 8, Number 4—April 2002
Genetic and Antigenic Analysis of the First A/New Caledonia/20/99-like H1N1 Influenza Isolates Reported in the Americas
|Primer/nt position||Sequence||% GCa||Tmb(°C)|
aGC= (Guanosine (G) + Cytidine (C))/ (Adenosine (A)+ Thymidine (T)+ Guanosine (G) + Cytidine (C)) x 100
bTm= 81.5 + 16.6 * log [Na+] + 41 * (#G + #C)/length – 500/length where [Na+]= 0.1M.
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