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Volume 9, Number 1—January 2003
Dispatch

Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

Catalina López-Saucedo*, Jorge F. Cerna*, Nicolas Villegas-Sepulveda*, Rocío Thompson*, F. Raul Velazquez†, Javier Torres†, Phillip I. Tarr‡§, and Teresa Estrada-García*Comments to Author 
Author affiliations: *Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., México; †Hospital de Pediatría, México D.F., México; ‡Children’s Hospital and Regional Medical Center, Seattle, Washington, USA; §University of Washington School of Medicine, Seattle, Washington, USA

Main Article

Table 2

Diarrheagenic Escherichia coli isolates in patient and food samplesa

Samples Diarrheagenic E. coli group Identified genes No. positive strains/ no. tested Other pathogens
isolated CFU/gram
food
Stools
Patient 1
 STEC
 stx1,eae A
 5/5
 none
Patient 2
 STEC
 stx2
 5/5
 none
Patient 3
 ETEC
 lt
 2/5
 none
Patient 4
 STEC
 stx 2
 2/9
 none
Patient 5
 STEC
 stx 2
 1/5
 none
Patient 6
 EIEC
 ial
 1/5
 none
Patient 7
 ETEC
 lt
 5/5
 Shigella flexneri
Patient 8
 ETEC
 st
 2/5
 S. sonnei
Patient 9
 EPEC
 bfpA, eaeA
 1/5
 S. sonnei
Patient 10
 ETEC
 lt
 1/5
 Rotavirus, S. sonnei
Patient 11
 STEC
 stx1, eae A
 1/10
 Rotavirus
Food
Green sauce
 ETEC
 lt, st
 5/5
8.0 X 102
Green sauce
 ETEC
 lt, st
 5/5
1.3 X 105
Raw cabbage
 STEC
 stx1, stx2
 2/5
2.6 X 105
Green sauce
 ETEC
 st
 1/5
2.6 X 104
Green sauce
 EIEC
 ial
 1/5
6.0 X 102
Raw coriander
 EIEC
 ial
 1/5
1.8 X 105
Raw lettuce EIEC ial 1/5 8.2 X 104

aSTEC, Shiga-toxin–producing Escherichia coli; ETEC, enterotoxigenic E. coli; EIEC, enteroinvasive E. coli; EPEC, enteropathogenic E. coli.

Main Article

Page created: December 07, 2010
Page updated: December 07, 2010
Page reviewed: December 07, 2010
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