Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 10, Number 10—October 2004
Research

Escherichia coli and Community-acquired Gastroenteritis, Melbourne, Australia

Roy M. Robins-Browne*†Comments to Author , Anne-Marie Bordun*†, Marija Tauschek*, Vicki R. Bennett-Wood*†, Jacinta Russell†, Frances Oppedisano†, Nicole A. Lister*, Karl A. Bettelheim*, Christopher K. Fairley*, Martha I. Sinclair‡, and Margaret E. Hellard‡1
Author affiliations: *University of Melbourne, Melbourne, Victoria, Australia; †Murdoch Childrens Research Institute, Melbourne, Victoria, Australia; ‡Monash University, Melbourne, Victoria, Australia

Main Article

Table 5

Characteristics of 22 isolates of atypical enteropathogenic Escherichia coli identified during this study

Strain Sourcea Serotype Intimin type Result of PCRb
Adhesion patterna FAS assay HEp-2 cell invasionc
bfpA aggR efa1 astA lpfD
W7 N O161:H40 β NA 0.01
W85-2 N OR:H- γ AA + 0.02
W114 N O107:H8 ι IA + 0.02
W143 N Ont:H5 ε IA + < 0.01
W145 N O55:H6 γ + + AA + 0.03
W154 N O139:H14 β AA + < 0.01
W185 N Ont:H21 θ IA + 0.02
W208 N Ont:H4 β IA + 0.08
W761 N O124:H40 λ NA 0.07
W902 N O125:H6 α2 IA + 0.06
W914 N O125:H6 α AA + 0.07
W1040 G O15:Hnt β + + LAL + 0.10
W1056 G O55:H7 γ + + IA + 0.77
W1068 G O51:H49 α AA + < 0.01
W1082 G Ont:H6 β AA + 0.01
W1092 G OR:H- η/ε + IA + < 0.01
W1108 G O172:H4 θ IA + 0.03
W1118 G O126:H6 α IA + 0.01
W1120 G Ont:H34 α IA + 0.01
W1134 G Ont:H6 θ NA 0.01
W1585 G Ont:H40 θ NA 0.02
W1706 G Ont:H6 α AA + 0.01
E128012 C O114:H2 β + + IA + 0.32

aN, no symptoms; G, gastroenteritis; C, control strain; Ont, O nontypable (O1–O181); Hnt, H nontypable (H1–H56); NA, nonadherent; AA, aggregative adherence pattern; IA, indeterminate adherence pattern; LAL, localized-like adherence pattern; FAS, fluorescent actin staining.
bPCR, polymerase chain reaction; +, positive; –, negative.
cData are the number of bacteria recovered from HEp-2 cells after treatment with gentamicin as a percentage of the total number of cell-associated bacteria and are the mean of two separate assays.

Main Article

1Current affiliation: Burnet Institute, Melbourne, Victoria, Australia.

Page created: April 11, 2011
Page updated: April 11, 2011
Page reviewed: April 11, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external