Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study

Ultrastructural Characterization of SARS Coronavirus

Cynthia S. Goldsmith*, Kathleen M. Tatti*, Thomas G. Ksiazek*, Pierre E. Rollin*, James A. Comer*, William W. Lee*, Paul A. Rota*, Bettina Bankamp*, William J. Bellini*, and Sherif R. Zaki*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Figure 4

Immunogold and in situ hybridization (ISH) labeling of severe acute respiratory syndrome–associated coronavirus–infected cells. A) Cytoplasmic area that is relatively free of organelles (arrow). B) At higher magnification, these regions are shown to consist of ribosomelike and filamentous structures. Within these regions, C) viral proteins are detected by immunolabeling, using hyperimmune mouse ascitic fluid (12 nm gold), and D) ultrastructural ISH detects viral mRNA, genRNA, or both, by using a

Figure 4. Immunogold and in situ hybridization (ISH) labeling of severe acute respiratory syndrome–associated coronavirus–infected cells. A) Cytoplasmic area that is relatively free of organelles (arrow). B) At higher magnification, these regions are shown to consist of ribosomelike and filamentous structures. Within these regions, C) viral proteins are detected by immunolabeling, using hyperimmune mouse ascitic fluid (12 nm gold), and D) ultrastructural ISH detects viral mRNA, genRNA, or both, by using a pool of riboprobes (6 nm gold). Bars, A,1 μm; B–D, 100 nm.

Main Article

Page created: January 31, 2011
Page updated: January 31, 2011
Page reviewed: January 31, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external