Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 6, Number 1—February 2000
Dispatch

Molecular Genetic Evidence of a Novel Morbillivirus in a Long-Finned Pilot Whale (Globicephalus melas)

Jeffery K. Taubenberger*Comments to Author , Mark M. Tsai*, T. Joy Atkin*, Thomas G. Fanning*, Amy E. Krafft*, R.B. Moeller*, S.E. Kodsi†, M.G. Mense†, and Thomas P. Lipscomb*
Author affiliations: *Armed Forces Institute of Pathology, Washington, D.C., USA; †Walter Reed Army Institute of Research, Washington, D.C., USA

Main Article

Figure 2

Neighbor-joining analyses of partial P and N gene sequences with branch distances as shown. Analyses were performed with MEGA, version 1.01 (13). For the P gene, a 378 nucleotide fragment was amplified (7,8) using the following primers: 5'-CGGAG ACCGAGTCTTCATT-3' (forward) and 5'-ATTGGGTTGC ACCACTTG TC-3' (reverse), corresponding to nucleotides 2190 to 2567 as aligned to the measles virus P gene (Edmonston strain). For the N gene, a 230-nucleotide fragment was amplified using the following prime

Figure 2. Neighbor-joining analyses of partial P and N gene sequences with branch distances as shown. Analyses were performed with MEGA, version 1.01 (13). For the P gene, a 378 nucleotide fragment was amplified (7,8) using the following primers: 5'-CGGAG ACCGAGTCTTCATT-3' (forward) and 5'-ATTGGGTTGC ACCACTTG TC-3' (reverse), corresponding to nucleotides 2190 to 2567 as aligned to the measles virus P gene (Edmonston strain). For the N gene, a 230-nucleotide fragment was amplified using the following primers: 5'-CCHAGRATYGCTGAAATGATHTGTGA-3' (forward) and 5'-AACTTG TTCTGRATWGAGTTYTC-3' (reverse), corresponding to nucleotides 849 to 1078, as aligned to the measles virus N gene (Edmonston strain, GenBank accession number Z66517). RT-PCR and sequence analysis were performed as described (7,8).

Main Article

References
  1. Kennedy  S. Morbillivirus infections in aquatic mammals. J Comp Pathol. 1998;119:20125. DOIPubMedGoogle Scholar
  2. Lipscomb  TP, Schulman  FY, Moffett  D, Kennedy  S. Morbilliviral disease in Atlantic bottlenose dolphins (Tursiops truncatus) from the 1987-1988 epizootic. J Wildl Dis. 1994;30:56771.PubMedGoogle Scholar
  3. Schulman  FY, Lipscomb  TP, Moffett  D, Krafft  AE, Lichy  JH, Tsai  MM, Reevaluation of the 1987-88 Atlantic coast bottlenose dolphin (Tursiops truncatus) mortality event with histologic, immunohistochemical, and molecular evidence for a morbilliviral etiology. Vet Pathol. 1997;34:28895. DOIPubMedGoogle Scholar
  4. Osterhaus  ADME, Groen  J, Spijkers  HEM, Broeders  HWJ, UytdeHaag  FGCM, de Vries  P, . Mass mortality in seals caused by a newly discovered morbillivirus. Vet Microbiol. 1990;23:34350. DOIPubMedGoogle Scholar
  5. Kennedy  S, Smyth  JA, Cush  PF, McAliskey  M, McCullough  SJ, Rima  BK. Seven histopathologic and immunocytochemical studies of distemper in harbor porpoises. Vet Pathol. 1991;28:17. DOIPubMedGoogle Scholar
  6. Domingo  M, Vilafranca  M, Visa  J, Prats  N, Trudgett  AL, Visser  I. Evidence of chronic morbillivirus infection in the Mediterranean striped dolphin (Stenella coeruleoalba). Vet Microbiol. 1995;44:22939. DOIPubMedGoogle Scholar
  7. Krafft  AE, Lichy  JH, Lipscomb  TP, Klaunberg  BA, Kennedy  S, Taubenberger  JK. Postmortem diagnosis of morbillivirus infection in bottlenose dolphins (Tursiops truncatus) in the Atlantic and Gulf of Mexico epizootics by a polymerase chain reaction-based assay. J Wildl Dis. 1995;31:4105.PubMedGoogle Scholar
  8. Lipscomb  TP, Kennedy  S, Moffett  D, Krafft  AE, Klaunberg  BA, Lichy  JH, Morbilliviral epizootic in Atlantic bottlenose dolphins of the Gulf of Mexico. J Vet Diagn Invest. 1996;8:28390.PubMedGoogle Scholar
  9. Reidarson  TH, McBain  J, House  C, King  DP, Stott  JL, Krafft  AE, Morbillivirus infection in stranded common dolphins (Delphinus delphis) from the Pacific Ocean. J Wildl Dis. 1998;34:7716.PubMedGoogle Scholar
  10. Barrett  T, Visser  IKG, Mamaev  L, Goatley  L, van Bressem  M-F, Osterhaus  ADME. Dolphin and porpoise morbilliviruses are genetically distinct from phocine distemper virus. Virology. 1993;193:10102. DOIPubMedGoogle Scholar
  11. Taubenberger  JK, Tsai  MM, Krafft  AE, Lichy  JH, Reid  AH, Schulman  FY, Two different morbilliviruses implicated in bottlenose dolphin epizootics. Emerg Infect Dis. 1996;2:2136. DOIPubMedGoogle Scholar
  12. Swofford  DL. PAUP: Phylogenetic Analysis Using Parsimony, version 3.1.1 (Illinois Natural History Survey, Champaign, Illinois; 1991.
  13. Kumar  S, Tamura  K, Nei  M. Molecular evolutionary genetics analysis, version 1.01. Pennsylvania State University, University Park, Pennsylvania; 1993.
  14. Duignan  PJ, House  C, Geraci  JR, Duffy  N, Rima  BK, Walsh  MT, Morbillivirus infection in cetaceans of the western Atlantic. Vet Microbiol. 1995;44:2419. DOIPubMedGoogle Scholar
  15. Duignan  PJ, House  C, Geraci  JR, Early  G, Copland  HG, Walsh  MT, Morbillivirus infection in two species of pilot whales (Globicephala sp.) from the western Atlantic. Mar Mamm Sci. 1995;11:15062. DOIGoogle Scholar

Main Article

Page created: May 09, 2011
Page updated: May 09, 2011
Page reviewed: May 09, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external