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Volume 9, Number 3—March 2003
Dispatch

Isolation of Escherichia coli O157:H7 from Intact Colon Fecal Samples of Swine1

Ingrid Feder*Comments to Author , F. Morgan Wallace*, Jeffrey T. Gray†, Pina Fratamico*, Paula J. Fedorka-Cray†, Rachel A. Pearce‡, Jeffrey E. Call*, Richard Perrine*, and John B. Luchansky*
Author affiliations: *U.S. Department of Agriculture, Wyndmoor, Pennsylvania, USA; †U.S. Department of Agriculture, Athens, Georgia, USA; ‡National Food Centre, Dublin, Ireland

Main Article

Table

Characterization of Escherichia coli O157:H7 and non-H7 isolates recovered from 305 swine fecal colon samplesa,b

Collection date Swine E. coli O157
isolate no. E. coli O157 positive colon samples/total colon samples collected Colon
ref.
no. No. of isolates recovered from sample PCR
characteristics E. coli O157 ribotyping PFGE type Antimicrobial resistance patternb
Feb. 16, 2001
 1
 2/5
 1
 1
 rfbO157, fliCH7, stx1, eae, hly933
 H7
 1
 Pan-sensitive
2
2
 1
 rfbO157, fliCH7, stx1, eae, hly933
 H7
 1
 Pan-sensitive
Mar. 8, 2001
 No isolates
 0/20
Mar. 22, 2001
 No isolates
 0/40
Apr. 20, 2001
 3
 3/40
 3
 1
 rfbO157
 Non-H7
 3
 kan, strept, sulfa, tet
4
4
 1
 rfbO157
 Non-H7
 3
 kan, strept, sulfa, tet
5
5
 1
 rfbO157
 Non-H7
 3
 kan, sulfa, tet
May 4, 2001
 6–11
 4/50
 6
 6
 rfbO157, fliCH7, stx1, stx2, eae
 H7
 2
 Pan-sensitive
12–16
7
 5
 rfbO157, fliCH7, stx1, stx2, eae
 H7
 2
 Pan-sensitive, except isolate no. 15 resistant to strept
17–25
8
 9
 rfbO157, fliCH7, stx1, stx2, eae
 H7
 2
 Pan-sensitive
26,27
9
 2
 rfbO157, fliCH7, stx1, stx2, eae
 H7
 2
 Pan-sensitive
May 16, 2001
 28
 9/50
 10
 1
 rfbO157
 Non-H7
 4
 Pan-sensitive
29
11
 1
 rfbO157
 Non- H7
 4
 tet
30
12
 1
 rfbO157
 Non-H7
 4
 tet
31
13
 1
 rfbO157
 Non-H7
 4
 strept, tet
32
14
 1
 rfbO157
 Non- H7
 4
 tet
33
15
 1
 rfbO157
 Non-H7
 4
 tet
34
16
 1
 rfbO157
 Non- H7
 4
 tet
35
17
 1
 rfbO157
 Non- H7
 4
 tet
36
18
 1
 rfbO157
 Non-H7
 4
 tet
June 20, 2001
 No isolates
 0/50
July 10, 2001
 No isolates
 0/50
Total=36 18/305 18

a Each isolate listed in the following table represents an isolate from an individual colon sample.
bFeb., February; Mar., March; Apr., April; ref., reference; PCR, polymerase chain reaction; PFGE, pulsed-field gel electrophoresis; kan, kanamycin; strept, streptomycin; sulfa, sulfamethoxazole; tet, tetracycline.

Main Article

1Presented in part at the 102nd Annual Meeting of the American Society of Microbiologists, Salt Lake City, Utah, May 2002.

Page created: December 07, 2010
Page updated: December 07, 2010
Page reviewed: December 07, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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