Volume 9, Number 9—September 2003
Research
Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome
Table
No. of patients (%) | Human metapneumovirus F-gene sequence detected by RT-PCR |
|||
---|---|---|---|---|
Nasopharyngeal aspirate | HEp-2 cells | LLC-MK2 cells | MDCK cells | |
1 (4.0) |
Positive |
Positive |
Positive |
Positive |
1 (4.0) |
Positive |
Positive |
Positive |
Negative |
1 (4.0) |
Negative |
Positive |
Positive |
Negative |
1 (4.0) |
Negative |
Positive |
Negative |
Positive |
18 (72.0) |
Negative |
Positive |
Negative |
Negative |
3 (12.0) | Negative | Negative | Positive | Negative |
aRT-PCR, reverse transcription-polymerase chain reaction; Hep-2, human laryngeal carcinoma monolayer; LLC-MK2, rhesus monkey kidney monolayer; MDCK, Mardin Darby Canine Kidney monolayer; BGM, Buffalo green monkey kidney monolayer.
bThe human metapneumovirus RT-PCR results for all human embryonic lung fibroblast, BGM and Vero cell cultures were negative.
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