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Volume 12, Number 9—September 2006
Letter

Fluoroquinolone-resistant Streptococcus pneumoniae

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To the Editor: In pneumococci, resistance to fluoroquinolones is associated with chromosomal mutations in the quinolone-resistance–determining regions (QRDR) of type II topoisomerase enzymes, predominantly gyrA and parC. Several mutations have been described in these enzymes, but only a few have been shown by in vitro studies to confer resistance: S81F or Y, C, or I and E85K in gyrA; E474K in gyrB; A63T, S79F or Y or L and D83G or N in parC; and E474K and D435N or H in parE (15). Other frequently described mutations are K137N in parC and I460V in parE, which appear to not contribute to fluoroquinolone resistance because they are commonly found in susceptible strains, and no evidence exists for their conferring fluoroquinolone resistance in vitro. We describe here a pneumococcal strain that was isolated from a 66-year-old white man with chronic obstructive pulmonary disease (COPD).

The patient was admitted to the hospital with a presumed exacerbation of COPD. He had been discharged from the hospital 2 days earlier, having recovered from a similar manifestation of this disease. His treatment history was 250 mg/day oral levofloxacin for 7 days while in the hospital and levofloxacin for 10 days as an outpatient for a similar lower respiratory tract infection 3 months earlier.

On this second admission he was given levofloxacin, 250 mg intravenously, once a day. He was treated with a low dosage because he was in renal failure. The patient continued to worsen and was transferred to the intensive care unit, where ceftriaxone, 1 g intravenously once a day, was given along with levofloxacin. He improved on the combination therapy and was discharged without sequelae.

Cultures of the patient's blood and sputum grew Streptococcus pneumoniae. The isolate from blood was resistant to levofloxacin (MIC 8 mg/L) and ciprofloxacin (MIC 8 mg/L), yet susceptible to gatifloxacin (MIC 1 mg/L) and ceftriaxone (MIC 0.38 mg/L), with intermediate resistance to penicillin (MIC 1.5 mg/L). The resistant isolate was of serotype 6A and of multilocus sequence type 376, which is the North Carolina6A-23 clone (http://www.sph.emory.edu/PMEN/index.html).

Efflux testing that compared the ciprofloxacin MICs in the presence and absence of reserpine (10mg/L) showed no evidence of an overexpressed efflux pump. We sequenced the QRDRs (gyrA, gyrB, parC, parE) and the entire gyrA and parC genes of the resistant strain isolated from blood by using previously described primers (2). Sequencing showed a S79Y mutation in parC and a Q118K (CAA→AAA) mutation in gyrA. Sequencing of the entire gyrA and parC genes confirmed that no additional amino acid substitutions were outside the QRDRs. The entire gyrA gene PCR product was transformed directly into the susceptible pneumococcal reference strain R6 by a standard transformation protocol (4). Transformants were selected on plates containing increasing concentrations of ciprofloxacin and, in a second step, were transformed with the entire parC gene of the resistant strain.

The ciprofloxacin and levofloxacin MICs of R6 transformed with the gyrA gene of the resistant isolate containing the new Q118K mutation were 4 and 2 mg/L, respectively. After additional transformation of these transformants with parC of the resistant isolate containing the S79Y mutation, the selected double transformants exhibited the same MICs as the original clinical isolate (8 mg/L for ciprofloxacin and levofloxacin). The transformation of parC alone conferred an intermediate increase in the MICs (ciprofloxacin 2 mg/L, levofloxacin 4 mg/L). All transformants were confirmed by sequencing.

To determine the biologic cost associated with the different resistance mutations in vitro, each fluoroquinolone-resistant mutant was competed against the fluoroquinolone-susceptible parent strain R6 (with an independent streptomycin resistance marker) as described by Johnson et al. (6). The outcome was evaluated as the change in the ratios of the competing strains as a function of the number of generations. Each competition was performed in triplicate by using independent starting cultures of each competing strain. Compared with the wild-type R6 strain, the relative fitness values for the gyrA, parC, and double mutants were 1.06, 1.03, and 0.93, respectively.

These data indicate that a single mutation in either parC or gyrA does not impose a substantial fitness burden. In contrast, the double-mutation parC S79Y and gyrA Q118K was associated with a slower growth rate. Similar results of relative fitness for single (parC S79Y and gyrA S81F) and double mutations were observed by Gillespie et al. (7).

Development of resistance to fluoroquinolones is a stepwise process, involving spontaneous mutations in the genes encoding the target enzymes DNA gyrase and the topoisomerase IV. Mutants with mutations in 1 of the enzymes are estimated to arise at a frequency of 1 to 10-7 (1). Therefore, fluoroquinolone resistance due to selection of spontaneous mutants during treatment may be related to the number of bacterial cells in the population under selective pressure. Patients with COPD are frequently colonized by high bacterial loads. COPD has been identified in several recent studies as an independent risk factor for fluoroquinolone resistance (8,9). Low doses of fluoroquinolones may also lead to an increased risk for resistance selection (10). Because the Q118K mutation has not been previously described, this new mutation was probably selected by the current or antecedent treatments rather than by an infection with a resistant widely disseminated clone.

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Acknowledgment

Mathias W.R. Pletz's work was supported by a scholarship from the German Research Foundation (Deutsche Forschungsgemeinschaft) and CAPNETZ.

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Mathias W. Pletz*†1Comments to Author , Randolph V. Fugit‡1, Lesley McGee*, Jeffery J. Glasheen§, Darcie L. Keller¶, Tobias Welte†, and Keith P. Klugman*#
Author affiliations: *Emory University Rollins School of Public Health, Atlanta, Georgia, USA; †Hannover Medical School, Hannover, Germany; ‡Denver Veterans Affairs Medical Center, Denver, Colorado, USA; §University of Colorado Health Sciences Center, Denver, Colorado, USA; ¶University of Missouri-Kansas City School of Medicine, Kansas City, Missouri, USA; #Emory University School of Medicine, Atlanta, Georgia, USA

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References

  1. Gillespie  SH, Voelker  LL, Ambler  JE, Traini  C, Dickens  A. Fluoroquinolone resistance in Streptococcus pneumoniae: evidence that gyrA mutations arise at a lower rate and that mutation in gyrA or parC predisposes to further mutation. Microb Drug Resist. 2003;9:1724. DOIPubMedGoogle Scholar
  2. Pan  XS, Ambler  J, Mehtar  S, Fisher  LM. Involvement of topoisomerase IV and DNA gyrase as ciprofloxacin targets in Streptococcus pneumoniae. Antimicrob Agents Chemother. 1996;40:23216.PubMedGoogle Scholar
  3. Korzheva  N, Davies  TA, Goldschmidt  R. Novel Ser79Leu and Ser81Ile substitutions in the quinolone resistance-determining regions of ParC topoisomerase IV and GyrA DNA gyrase subunits from recent fluoroquinolone-resistant Streptococcus pneumoniae clinical isolates. Antimicrob Agents Chemother. 2005;49:247986. DOIPubMedGoogle Scholar
  4. Weigel  LM, Anderson  GJ, Facklam  RR, Tenover  FC. Genetic analyses of mutations contributing to fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae. Antimicrob Agents Chemother. 2001;45:351723. DOIPubMedGoogle Scholar
  5. Perichon  B, Tankovic  J, Courvalin  P. Characterization of a mutation in the parE gene that confers fluoroquinolone resistance in Streptococcus pneumoniae. Antimicrob Agents Chemother. 1997;41:11667.PubMedGoogle Scholar
  6. Johnson  CN, Briles  DE, Benjamin  WH Jr, Hollingshead  SK, Waites  KB. Relative fitness of fluoroquinolone-resistant Streptococcus pneumoniae. Emerg Infect Dis. 2005;11:81420.PubMedGoogle Scholar
  7. Gillespie  SH, Voelker  LL, Dickens  A. Evolutionary barriers to quinolone resistance in Streptococcus pneumoniae. Microb Drug Resist. 2002;8:7984. DOIPubMedGoogle Scholar
  8. Ho  PL, Yung  RW, Tsang  DN, Que  TL, Ho  M, Seto  WH, Increasing resistance of Streptococcus pneumoniae to fluoroquinolones: results of a Hong Kong multicentre study in 2000. J Antimicrob Chemother. 2001;48:65965. DOIPubMedGoogle Scholar
  9. Vanderkooi  OG, Low  DE, Green  K, Powis  JE, McGeer  A. Predicting antimicrobial resistance in invasive pneumococcal infections. Clin Infect Dis. 2005;40:128897. DOIPubMedGoogle Scholar
  10. Andes  D, Anon  J, Jacobs  MR, Craig  WA. Application of pharmacokinetics and pharmacodynamics to antimicrobial therapy of respiratory tract infections. Clin Lab Med. 2004;24:477502. DOIPubMedGoogle Scholar

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Cite This Article

DOI: 10.3201/eid1209.051400

1These authors contributed equally to this paper.

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Mathias W.R. Pletz, Department of Respiratory Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, Hannover, 30625, Germany

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Page created: November 17, 2011
Page updated: November 17, 2011
Page reviewed: November 17, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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