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Volume 13, Number 10—October 2007
Dispatch

Rapid Field Immunoassay for Detecting Antibody to Sin Nombre Virus in Deer Mice

Tony Schountz*Comments to Author , Charles H. Calisher†, Tiffany R. Richens†, Audrey A. Rich*, Jeffrey B. Doty†, Mark T. Hughes†, and Barry J. Beaty†
Author affiliations: *University of Northern Colorado, Greeley, Colorado, USA; †Colorado State University, Fort Collins, Colorado, USA;

Main Article

Table

Comparison of results of PAGEIA and standard EIA for detection of antibody to Sin Nombre virus (SNV) in blood samples from 40 deer mice captured in southwest Colorado, 2006*

Accession no. Field PAGEIA
score† Laboratory PAGEIA 
OD‡ Laboratory PAGEIA
score§ Standard EIA 
OD¶ Standard EIA 
score#
HA-2548 3+ 1.254 Pos 0.903/0.066 Pos
HA-2552 4+ 2.406 Pos 1.083/0.113 Pos
HA-2554 4+ 1.788 Pos 1.395/0.058 Pos
HA-2558 4+ 2.383 Pos 1.462/0.055 Pos
HA-2560 4+ 1.913 Pos 1.378/0.086 Pos
HA-2564 0 0.001 Neg 0.327/0.055 Pos
HA-2565 1+ 0.236 Pos 0.715/0.046 Pos
HA-2567 4+ 2.123 Pos 1.485/0.080 Pos
HB-2604 4+ 2.065 Pos 1.161/0.067 Pos
HB-2608 2+ 0.855 Pos 0.653/0.095 Pos
HB-2612 1+ 0.282 Pos 1.136/0.071 Pos
HB-2616 1+ 0.311 Pos 0.458/0.001 Pos
HB-2617 2+ 0.517 Pos 0.819/0.008 Pos
HB-2618 2+ 0.494 Pos 1.085/0.009 Pos
HB-2622 3+ 1.254 Pos 1.519/0.029 Pos
HB-2628 1+ 0.493 Pos 0.220/0.082 Neg
HB-2630 3+ 1.609 Pos 0.681/0.008 Pos
HA-2570 4+ 1.970 Pos 0.389/0.024 Pos
HA-2578 4+ 2.101 Pos 1.185/0.017 Pos
HB-2642 4+ 2.784 Pos 1.294/0.063 Pos
HA-2601 4+ 2.699 Pos 0.921/0.121 Pos
HA-2609 2+ 0.608 Pos 0.228/0.042 Neg
HA-2612 4+ 2.482 Pos 1.072/0.085 Pos
HA-2616 1+ 0.331 Pos 0.076/0.059 Neg
HB-2681 3+ 0.977 Pos 1.392/0.048 Pos
HB-2682 3+ 1.095 Pos 1.326/0.042 Pos
HA-2634 4+ 3.010 Pos 0.863/0.014 Pos
HA-2647 4+ 2.824 Pos 0.720/0.023 Pos
HA-2666 4+ 2.682 Pos 0.477/0.028 Pos
HB-2706 1+ 0.836 Pos 0.324/0.032 Pos
HB-2710 2+ 0.664 Pos 0.155/0.035 Neg
HB-2712 3+ 1.599 Pos 0.345/0.033 Pos
HB-2717 1+ 1.098 Pos 0.293/0.027 Pos
HB-2720 3+ 2.581 Pos 0.630/0.039 Pos
TS-0830–6 2+ 0.889 Pos 0.799/0.097 Pos
TS0830–7 1+ 0.000 Neg 0.030/0.033 Neg
TS-0830–8 3+ 2.014 Pos 0.800/0.024 Pos
TS-0830–9 4+ 1.949 Pos 0.884/0.054 Pos
TS-0830–18 4+ 2.112 Pos 1.180/0.021 Pos
TS-0830–20 3+ 1.427 Pos 0.820/0.072 Pos

*PAGEIA, protein-A/G horseradish peroxidase enzyme-linked immunosorbent assay; EIA, enzyme immunoassay; OD, optical density; Pos, positive; Neg, negative.
†Field scores were based upon visual inspection without instrumentation, with 0 as negative, 1+ as very weak, 2+ as weak, 3+ as strong, and 4+ as very strong, relative to positive and negative control samples.
‡Field-collected samples were retested by PAGEIA under laboratory conditions and the OD reported here. The instrument was blanked on the negative control sample.
§OD >0.200 above the negative control was considered positive.
¶For laboratory EIA, OD was recorded for diluted (1:100) blood with both SNV antigen (numerator) and control antigen (denominator).
#A sample tested with SNV antigen and having an OD >0.300 was considered positive if the OD of that sample with the control antigen was <0.150. In regard to sample HBV-2717, the OD of the laboratory EIA with antigen was 0.293, very near the acceptable minimum, and the background was 0.027, which is very low; this sample was considered provisionally positive.

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