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Volume 14, Number 2—February 2008

Experimental Infection and Natural Contact Exposure of Dogs with Avian Influenza Virus (H5N1)

Matthias Giese*, Timm C. Harder*, Jens P. Teifke*, Robert Klopfleisch*, Angele Breithaupt*, Thomas C. Mettenleiter*, and Thomas W. Vahlenkamp*Comments to Author 
Author affiliations: *Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany;

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Course of experimental influenza virus (H5N1) infection of dogs and in-contact animals*

Dog no. EID50 Body temperature (dpi)
Pharyngeal swab (dpi)
(dpi) Antibodies
0 2 4 6 0 2 4 6 HI ELISA
1 106 38.5 39.2 38.8 38.8 + 10 (+)†
2 106 38.4 39.7 39.1 38.8 + 10 (+)‡
3 106 38.5 39.3 38.6 38.4 + 21 16 +
4 106 38.7 39.3 39.1 38.6 21
5 (dog contact) 0 38.3 38.4 38.0 38.7 21
6 (cat contact) 0 38.4 38.2 38.4 38.5 21
7 (cat contact) 0 38.3 38.5 38.6 38.8 21
8 (cat contact) 0 38.4 38.5 38.2 38.3 21
9 (neg. control) 0 38.4 38.2 38.7 37.8 21
10 (neg. control) 0 38.3 38.6 38.8 38.0 21

*Elevated body temperatures (above 39°C) are underlined. Real time reverse transcription–PCR results are given as – (cycle threshold [ct] values >38) and + (ct values 31–38). Animal serum samples were examined by hemagglutination-inhibition (HI) using highly pathogenic influenza virus (H5N1) strain A/cat/Germany/R606/2006 (9) as antigen. Results are given as reciprocal titers. Competitive nucleoprotein ELISA results are given as – (inhibition <65%) and + (inhibition >65); EID50, 50% egg infectious dose; dpi, days postinfection.
†Serum from dog no. 1 on dpi 10 showed 39.4% inhibition.
‡Serum from dog no. 2 showed 21.6% inhibition.

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  1. Keawcharoen  J, Oraveerakul  K, Kuiken  T, Fouchier  RA, Amonsin  A, Payungporn  S, Avian influenza H5N1 in tigers and leopards. Emerg Infect Dis. 2004;10:218991.PubMedGoogle Scholar
  2. Klopfleisch  R, Wolf  PU, Uhl  W, Gerst  S, Harder  T, Starick  E, Distribution of lesions and antigen of highly pathogenic avian influenza virus A/swan/Germany/R65/06 (H5N1) in domestic cats after presumptive infection by wild birds. Vet Pathol. 2007;44:2618. DOIPubMedGoogle Scholar
  3. Leschnik  M, Weikel  J, Möstl  K, Revilla-Fernandez  S, Wodak  E, Bago  Z, Subclinical infection with avian influenza A (H5N1) virus in cats. Emerg Infect Dis. 2007;13:2437.PubMedGoogle Scholar
  4. Thanawongnuwech  R, Amonsin  A, Tantilertcharoen  R, Damrongwatanapokin  S, Theamboonlers  A, Payungporn  S, Probable tiger-to-tiger transmission of avian influenza H5N1. Emerg Infect Dis. 2005;11:699701.PubMedGoogle Scholar
  5. Songserm  T, Amonsin  A, Jam-on  R, Sae-Heng  N, Paryothorn  N, Payungporn  S, Fatal avian influenza A H5N1 in a dog. Emerg Infect Dis. 2006;12:17446.PubMedGoogle Scholar
  6. Amonsin  A, Songserm  T, Chutinimitkul  S, Jam-on  R, Sae-Heng  N, Pariyothorn  N, Genetic analysis of influenza A virus (H5N1) derived from domestic cat and dog in Thailand. Arch Virol. 2007;152:192533. DOIPubMedGoogle Scholar
  7. Butler  D. Thai dogs carry bird-flu virus, but will they spread it? Nature. 2006;439:773. DOIPubMedGoogle Scholar
  8. Mahardika  GN. Experts call for monitoring of cats, dogs for H5N1. Reuters Jakarta. February 1, 2007 [cited 2007 Feb 3]. Available from
  9. Weber  S, Harder  T, Starick  E, Beer  M, Werner  O, Hoffmann  B, Molecular analysis of highly pathogenic avian influenza virus of subtype H5N1 isolated from wild birds and mammals in northern Germany. J Gen Virol. 2007;88:5548. DOIPubMedGoogle Scholar
  10. Spackman  E, Senne  DA, Myers  TJ, Bulaga  LL, Garber  LP, Perdue  ML, Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol. 2002;40:325660. DOIPubMedGoogle Scholar
  11. Kuiken  T, Rimmelzwaan  GF, van Riel  D, van Amerongen  G, Baars  M, Fouchier  RAM, Avian H5N1 influenza in cats. Science. 2004;306:241. DOIPubMedGoogle Scholar
  12. Rimmelzwaan  GF, van Riel  D, Baars  M, Bestebroer  TM, van Amerongen  G, Fouchier  RAM, Influenza A virus (H5N1) infection in cats causes systemic disease with potential novel routes of virus spread within and between hosts. Am J Pathol. 2006;168:17683. DOIPubMedGoogle Scholar
  13. Crawford  PC, Dubovi  EJ, Castleman  WL, Stephenson  I, Gibbs  EP, Chen  L, Transmission of equine influenza virus to dogs. Science. 2005;310:4825. DOIPubMedGoogle Scholar

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