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Volume 17, Number 5—May 2011
Letter

Yersinia pestis DNA Sequences in Late Medieval Skeletal Finds, Bavaria

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To the Editor: We read with interest the report by Wiechmann et al. that, in the investigation of late medieval plague, partial sequencing of the Yersinia pestis pPCP1 plasmid yielded the observation of a 3-T homopolymeric tract which differed from the 5-T homopolymeric tract of the Orientalis Y. pestis CO92 type strain (1). This observation was unexpected because previous data from multispacer sequence typing and glp D gene sequencing yielded only the Orientalis biotype in cases of ancient plague (2).

Using suicide PCR (3), we therefore further investigated pPCP1 in 10 negative control dental pulp specimens and 60 specimens collected from 1 Justinian Orientalis plague site (2), 2 Black Death Orientalis sites, and 2 additional medieval plague sites. All negative controls remained negative; 14 (23%) of 60 plague specimens yielded a PCR product, and 7 interpretable sequences yielded a 3-T homopolymeric tract in all cases.

We further tested a Y. pestis isolate collection comprising 2 Antiqua, 6 Medievalis, and 4 Orientalis strains. No amplification was obtained in DNA-free PCR mix and 5 Y. enterocolitica–negative control isolates, whereas sequencing yielded a 3-T homopolymeric tract in all 12 Y. pestis isolates.

BLAST analysis (http://blast.ncbi.nlm.nih.gov/blast.cgi) indicated that the 5-T homopolymeric tract has been found only once in the Y. pestis CO92 strain (4) and in none of 22 modern and 11 ancient sequences (Table). This 5-T homopolymeric tract is therefore CO92 strain specific and not a marker for the Orientalis biotype. This pPCP1 plasmid sequence, located into a noncoding region of the 3′ extremity of the plasmid, is characterized by several homopolymeric tracts of poly (A) and poly (T), including the 1 herein investigated. Instability of the T-stretches has been reported in bacterial genomes (5) as being hot spots for mutations (5).

Therefore, in our assessment, the data reported for the late medieval Bavaria burial (1) do not support that deaths of persons buried in this site resulted from a non-Orientalis plague. Typing modern or ancient Y. pestis strains should not rely on poly (A) and poly (T) homopolymeric tracts sequencing.

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Acknowledgment

This study was funded by Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique 6236.

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Thi-Nguyen-Ny Tran, Didier Raoult, and Michel Drancourt
Author affiliations: Author affiliation: Université de la Méditerranée, Marseille, France

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References

  1. Wiechmann  I, Harbeck  M, Grupe  G. Yersinia pestis DNA sequences in late medieval skeletal finds, Bavaria. Emerg Infect Dis. 2010;16:18067.PubMedGoogle Scholar
  2. Drancourt  M, Roux  V, Dang  LV, Tran-Hung  L, Castex  D, Chenal-Francisque  V, Genotyping, Orientalis-like Yersinia pestis, and plague pandemics. Emerg Infect Dis. 2004;10:158592.PubMedGoogle Scholar
  3. Raoult  D, Aboudharam  G, Crubézy  E, Larrouy  G, Ludes  B, Drancourt  M. Molecular identification by “suicide PCR” of Yersinia pestis as the agent of medieval Black Death. Proc Natl Acad Sci U S A. 2000;97:128003. DOIPubMedGoogle Scholar
  4. Parkhill  J, Wren  BW, Thomson  NR, Titball  RW, Holden  MT, Prentice  MB, Genome sequence of Yersinia pestis, the causative agent of plague. Nature. 2001;413:5237. DOIPubMedGoogle Scholar
  5. Bechah  Y, El Karkouri  K, Mediannikov  O, Leroy  Q, Pelletier  N, Robert  C, Genomic, proteomic, and transcriptomic analysis of virulent and avirulent Rickettsia prowazekii reveals its adaptive mutation capabilities. Genome Res. 2010;20:65563. DOIPubMedGoogle Scholar

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Table

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Cite This Article

DOI: 10.3201/eid1705.101777

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In Response: We thank Tran et al. for their interest in our article (1). In it, we described the detection of several Yersinia pestis–specific plasmid pPCP1 DNA sequences in skeletal remains from a late medieval mass burial in Bavaria, Germany. In 1 of these sequence sections, we found a deviation from the reference sequence used (Y. pestis strain CO92 plasmid sequence AL109969.1). We did not further interpret this result because we agree with Tran et al. that typing of Y. pestis strains should not rely on poly (A) and poly (T) homopolymeric tract sequencing (2). As we have stated (1), further analyses of our material, including chromosomal markers (3,4) will be conducted to obtain clues as to the specific Y. pestis strain.

References

  1. Wiechmann  I, Harbeck  M, Grupe  G. Yersinia pestis DNA sequences in late medieval skeletal finds, Bavaria [letter]. Emerg Infect Dis. 2010;16:18067.PubMedGoogle Scholar
  2. Tran  T-N-N, Raoult  D, Drancourt  D. Yersinia pestis DNA sequences in late medieval skeletal finds, Bavaria [letter]. Emerg Infect Dis. 2011;17:9557.PubMedGoogle Scholar
  3. Morelli  G, Song  Y, Mazzoni  CJ, Eppinger  M, Roumagnac  P, Wagner  DM, Yersinia pestis genome sequencing identifies patterns of global phylogenetic diversity. Nat Genet. 2010;42:11403. DOIPubMedGoogle Scholar
  4. Haensch  S, Bianucci  R, Signoli  M, Rajerison  M, Schultz  M, Kacki  S, Distinct clones of Yersinia pestis caused the Black Death. PLoS Pathog. 2010;6:e1001134. DOIPubMedGoogle Scholar

Table of Contents – Volume 17, Number 5—May 2011

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Page created: August 19, 2011
Page updated: August 21, 2011
Page reviewed: August 21, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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